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| Simultaneous determination of phenolic acids and coumarins for quality control of Rhizoma et Radix Notopterygii.by HPLC-UV |
| Hits 2198 Download times 1893 Received:February 05, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.03.11 |
| Key Words
Rhizoma et Radix Notopterygii.;HPLC-UV;content determination |
| Author Name | Affiliation | E-mail | | YANG Xi | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GONG Mei-ling | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIN Hua | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] An HPLC-UV method was established to simultaneously determine the content of phenolic acids (neochlorogenic acid, Chlorogenic acid, Ferulic acid) and coumarins (Notopterol, Isoimperatorin) for the quality control of Rhizoma et Radix Notopterygii. [Methods] An Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with a guard column was used as the analytical column to separate the multi-ingredients. The mobile phase was acetonitrile and 0.2% (V/V) phosphoric acid solution. The flow rate was set at 1 mL/min. The injection volume was 5 μL and the eluent was measured at 330 nm. [Results] This method exhibited a good linear relationship, and the results of the method validation up to the requirements. [Conclusion] The established HPLC-UV assay could be used to determinate the content of Rhizoma et Radix Notopterygii for the quality control. |
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