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| Simultaneous determination of four components in Euphorbia Humifusa Willd. by UPLC |
| Hits 1824 Download times 1392 Received:March 01, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.03.12 |
| Key Words
UPLC;simultaneous determination;Euphorbia Humifusa Willd.;content |
| Author Name | Affiliation | E-mail | | FENG Guang-wei | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Qiannan Medical College for Nationalities, Duyun, Guizhou, 558000, China | | | LIU Zhi-dong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com | | QI Dong-li | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PI Jia-xin | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GU Xing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Qian | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HUANG Rui | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To establish an UPLC method for simultaneous determination four index components such as gallic acid, ellagic acid, quercetin and kaempferol in Euphorbia Humifusa Willd. [Methods] A chromatographic separation method was established with Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7μm) with methanol-acetonitrile (40:60, A)-0.2% formic acid (B) as mobile phases at the flow rate of 0.2 mL/min. The gradient elution was carried out as follows: 0~3 min, 5%A; 3~4 min, 5%~17%A;4~19min, 17%A;19~42 min, 17%~30%A; 42~50 min, 30%~34%A; 50~55 min, 34%~70%A. The detection wavelength was 270 nm at 0~4 min and 360 nm at 4~55 min, and the column temperature was 30℃. [Results] The four index components were well separated and each component had a good linear relationship in the liner range (r>0.999 7); and the recovery was in the range of 96.5%~102.9%. [Conclusion] The method is accurate, sensitive and repeatable for the comprehensive quality control of Euphorbia Humifusa Willd. |
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