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| Influence of Astragalus on expression of nuclear factor-κB induced by high glucose in human proximal renal tubular epithelial cells |
| Hits 2139 Download times 1606 Received:April 15, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.04.09 |
| Key Words
diabetic nephropathy;renal tubular epithelial cell;nuclear factor-κB;Astragalus |
| Author Name | Affiliation | E-mail | | MEI Sha-sha | Department of traditional Chinese medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | SONG En-feng | Department of traditional Chinese medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | songef@126.com | | XIANG Qiong | Department of traditional Chinese medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | |
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| Abstract
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| [Objective] To investigate the influence of Astragalus on expression of nuclear factor-κb(NF-κB) induced by high glucose in human proximal renal tubular epithelial cells(HK-2). [Methods] Human renal proximal tubular cells were cultured and divided into five groups: low glucose as control group; high glucose group; astragalus pretreatment group with concentration of 2 μg/mL, 20 μg/mL and 200 μg/mL. Each group has three samples. Culturing in 37 ℃ incubator for 24 hours, 48 hours and 72 hours, inverted microscope observe the cells. The expression level of NF-κB protein was detected by Western blotting. [Results] Compared with high glucose groups, the drug intervention groups grouped more better, expressed NF-κB protein level was lower（P<0.05), and the effect of astragalus were showed in dose-dependent manner. [Conclusion] High glucose may induce HK-2 cells to express NF-κB and decrease growth. Astragalus has protective effect to HK-2 cells by inhibiting HK-2 cells expression of NF-κB . |
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