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| LX-2 and HK-2 cell co-culture model and the influence of its cells |
| Hits 2202 Download times 2596 Received:April 25, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.05.09 |
| Key Words
renal tubular epithelial cell trans-differentiation;Transwell;LX-2 cell;HK-2 cell;cell co-culture |
| Author Name | Affiliation | | WANG Yao-guang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZENG You-jia | Shenzhen City Hospital of traditional Chinese medicine, Shenzhen 518033, China | | ZHOU Hui-jie | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish human hepatic stellate cells (LX-2) and renal tubular epithelial cells (HK-2) co-culture system theory model, research LX-2 cells influence on transdifferentiation of HK-2 cells.[Methods] By Transwell Chambers establish human hepatic stellate cells in vitro (LX-2 cells) and human renal tubular epithelial cells (HK)-2 cells indirectly co-culture system. By cellular immune histochemical method to detect α-SMA of HK-2 cells; With ELISA method to detect the TGF-beta 1 in cell cultures of the content; By RT-PCR method to detect of T beta RⅠmRNA, T beta R Ⅱ mRNA, Smad2 mRNA, Smad3 mRNA and Smad7 mRNA expression of HK-2 cell.[Results] Except the blank group, the two other groups on morphology can see HK-2 dye cell cytoplasm tan, different number of cells from cubic paving stone sample into spindle long strips, cell hypertrophy; HK-2 high expression of α-SMA cells (P<0.01); TGF-beta 1 content in the HK-2 cells supernatant fluid was obviously higher than blank group (P<0.01). TβRⅠ, Smad2 and Smad3 gene expression increase obviously, while TβRⅡ, Smad7 gene expression significantly lowered in HK-2 cells.[Conclusion] Transwell culture method can be induced to HK-2 cell trans-differentiation, its mechanism may be related to the TGF beta 1/Smad signaling pathways. |
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