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| Influence of processing time on Eucommia cortex and its pharmacokinetics study |
| Hits 2208 Download times 1819 Received:June 05, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.05.10 |
| Key Words
Eucommia;processing;LC-MS/MS;pharmacokinetics |
| Author Name | Affiliation | E-mail | | ZHANG Ying-yue | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAN Ya-ya | Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAO Jia | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Tao | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Er-wei | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | liuwei628@hotmail.com |
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| Abstract
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| [Objectives] To develop a set of LC-MS/MS method for the simultaneous determination the compound of geniposide, geniposidic acid, aucubin and pinoresinol diglucoside in the different processed barks of Eucommia and for the analysis of biolcgical samples which contain the compound of geniposide, geniposidic acid, aucubin and pinoresinol diglucoside from the rats' oral administration of the Eucommia.[Methods] The Eucommia was processed with saline water according to Chinese pharmacopoeia. In the parch processing course, samples were randomly collected at 0, 1, 2 and 4 h respectively. Validation of a high performance liquid chromatography tandem triple quadrupole mass spectrometry LC-MS/MS (QQQ) method, with the strychni prime as internal standard, was used for the determination of the four target compounds in rats' plasma to study the pharmacokinetics of Eucommia.[Results] The determination methods with good precision and stability were suitable for the assay of Eucommia in the biological samples. It is reasonable to control the process time for Eucommia at 2 hours.[Conclusion] Additionally, these constituents demonstrated good pharmacokinetic properties in the body. The pharmacokinetics parameters in our research provided with information for the clinical application. |
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