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| Experimental study of effect of Wuzi Jiangtang Formula on glucose and C-P of T2DM mice and DPP-4 activity |
| Hits 2300 Download times 112 Received:July 01, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.06.08 |
| Key Words
DPP-4;Wuzi Jiangtang Formula;Type 2 diabetes mellitus;GK rat |
| Author Name | Affiliation | E-mail | | ZHOU Xue-mei | North China University of Science and Technology, Tangshan 063000, China | | | DONG Yu-shan | North China University of Science and Technology, Tangshan 063000, China | | | LA Xiao-jin | North China University of Science and Technology, Tangshan 063000, China | | | TIAN Chun-yu | North China University of Science and Technology, Tangshan 063000, China | | | GAO Xiu-juan | North China University of Science and Technology, Tangshan 063000, China | | | HE Bao-ling | North China University of Science and Technology, Tangshan 063000, China | | | LIU Yu-jie | Health Bureau of Qianxi County of Tangshan City, Tangshan 064300, China | | | LI Ji-an | North China University of Science and Technology, Tangshan 063000, China | lnyy@vip.sina.com | | CHEN Zhen | Oriental Herbs Kft., Budapest 1174, Hungary | | | SHI Jia-sen | North China University of Science and Technology, Tangshan 063000, China | | | YUAN Li-li | North China University of Science and Technology, Tangshan 063000, China | | | QIU Ji-chao | North China University of Science and Technology, Tangshan 063000, China | |
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| Abstract
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| [Objective] To investigate the effect of Wuzi Jiangtang Formula on the effect of DPP-4,and study the possible mechanism of its hypoglycemic effect.[Methods] Select SPF male GK rats,the rat model of T2DM was established by feeding high fat for 4 weeks after adaptive feeding 1 week,detect blood glucose,FBG>11.1 mmol/L or 2 hPG>16.7 mmol/L was considered succeed model.And the T2DM rats were randomly divided into model group,metformin group,Wuzi Jiangtang Formula groups with high,medium and low dose respectively,normal group.The normal group and model group were administered intragastrically with pure water.Other groups were administered intragastrically with corresponding drugs for 8 weeks.The level of FBG was detected at the beginning of treatment,and at the ends of the 4th and 8th week.The rats were sacrificed after 8 weeks.The level of C-P were detected;Establish vitro screening model of DPP-4 inhibitor,and screen Wuzi Jiangtang Formula and its component drugs,which including Tusizi,Nvzhenzi,Suzi,Laifuzi and Chenqinzi.Select the Sitagliptin Phosphate Tablets as positive control drug.Then determine the absorbance (A),calculate the inhibition rate,and compare the difference of IC50.[Results] Wuzi jiangtang Formula can reduce the level FBG,2 hPG and C-P;IC50 of Wuzi jiangtang Formula is (6 667.754±2 119.759) mg/L,IC50 of Laifuzi is (382.554±7.749 6) mg/L,inhibition of DPP-4,IC50 of Tusizi is (843.391±135.23) mg/L.The inhibitory effect of DPP-4 of Cheqianzi,Suzi and Nvzhenzi is less than 10%.[Conclusion] Wuzi Jiangtang Formula can reduce the level FBG and 2 h PG,improve the islet β cell function.The inhibitory effect on DPP-4 is the possible mechanism,and Laifuzi,Tusizi are probably the main drugs play an important role in the inhibition of DPP-4. |
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