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| Effect of Danzhi decoction on serum levels of inflammatory factors in murine model with sequelae of pelvic inflammatory disease |
| Hits 1614 Download times 1304 Received:September 30, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2017.01.09 |
| Key Words
Sequelae of pelvic inflammatory disease;Danzhi Decoction;inflammatory factor |
| Author Name | Affiliation | E-mail | | LIANG Zhao | Beijing University of Chinese Medicine, Beijing 100029, China | | | LU Qiu-dan | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | JIN Zhe | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | jinzhe0231@163.com |
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| Abstract
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| [Objective] To investigate the effect of Danzhi Dection (DZD) on serum levels of interleukin-17A(IL-17A), interleukin-17F(IL-17F), monocyte chemoattractant protein-1(MCP-1) and macrophage inflammatory protein-2(MIP-2) in murine model with sequelae of pelvic inflammatory disease. To explore the pathogenesis of sequelae of pelvic inflammatory disease and explain the pharmacological action of Danzhi Dectionin treating SPID.[Methods] 48 BALB/c mice were randomly divided into normal control group, model group, aspirin (ASP) group[20.55 mg/(kg·d)], DZD low-dose group[7.91 mg/(kg·d)], DZD mid-dose group[15.82 mg/(kg·d)] and DZD high-dose group[31.64 mg/(kg·d)], 8 mice in each group. The mice of the model group, ASP group, DZD low-dose group, DZD mid-dose group and DZD high-dose group were given injected Chlamydia muridarum into the uterine cavity to induce SPID. After modeling of 6 weeks, each group were given medicine for 15 d. The mouse grimace scale(MGS), the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 of mice were detected.[Results] To compare with model group, the MGS, the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 were significantly decreased in ASP group, DZD low-dose group, DZD mid-dose group and DZD high-dose group(P<0.01). And the effect of DZD mid-dose group was better than other treatment groups(P<0.05).[Conclusion] SPID can enhance the expression levels of IL-17A, IL-17F, MCP-1 and MIP-2, Danzhi Dection can effective reduce the MGS, the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 in SPID mice. Danzhi Dection is the better choice to treat SPID. |
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