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| Effect of Guwei method on the IL-10 and IL-1β in the plasma of rats with skin abscess under the guidance of huchang theory |
| Hits 1773 Download times 1753 Received:October 15, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2017.01.10 |
| Key Words
"huchang" theory;"guwei" method;rat skin abscess;Hongzhongxiao;IL-10;IL-1β |
| Author Name | Affiliation | | ZHANG Zhao-hui | The Second Affilioded Hospital of Tianjin Univesity of Traditional Chinese Medicine, Tianjin 300193, China | | MA Jing | The Second Affilioded Hospital of Tianjin Univesity of Traditional Chinese Medicine, Tianjin 300193, China | | ZHU Chao-jun | The Second Affilioded Hospital of Tianjin Univesity of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Yang | Tianjin People's Hospital, Tianjin 300211, China | | XU Qiang | The Second Affilioded Hospital of Tianjin Univesity of Traditional Chinese Medicine, Tianjin 300193, China | | GUO Yue | Tianjin People's Hospital, Tianjin 300211, China | | ZHAO Li-kun | Tianjin People's Hospital, Tianjin 300211, China | | LIU Ting-ting | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Wan-ying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | JI Xiao-na | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] Effect of the method of "hoop circumference"(Hongzhongxiao) on the expression of IL-10 and IL-1β in the plasma of rats with skin abscess under the guidance of the theory of "defended field".[Methods] Forty rats were divided into blank group, model group, control group, pretreatment group and treatment group. In addition to the blank group, the other four groups of subcutaneous injection of Staphylococcus aureus caused by subcutaneous abscess model. The model group with normal saline treatment, the control group used Mupirocin Ointment dressing in the treatment group, pretreatment group in the injection of bacteria after 4 h of external Hong zhong xiao 2 mL dressing, the treatment group for external use hongzhong elimination tincture 2 mL dressing, drugs over a range of swelling range 1 cm, dressing was changed 1 times one day. In the treatment of 3 d, 7 d, 14 d, 18 d through the heart to take blood 2 mL, ELISA method to detect the expression of IL-10 and IL-1β in plasma.[Results] The level of IL-10 in each group was increased after 3 d, and there was significant difference between pretreatment group and blank group, suggesting that the early application of Hongzhongxiao increase the content of IL-10 in plasma. Compared with the other three groups, there were significant differences in the levels of IL-10 between pretreatment group and treatment group at the 7th day after the treatment. At the 7th day, the early intervention of the Hongzhongxiao and the later than scleroma formation intervention can increased the plasma IL-10 content. In the late stage of treatment (14~18 d), the limitations of the abscess, the group of plasma IL-10 content of no difference.After 3 days treatment, the levels of IL-1β in the model group, the control group, the pretreatment group and the treatment group were statistically significant compared with the blank group (P<0.01). There was no significant difference between model group, control group, pretreatment group and treatment group in IL-1β level (P>0.05). Compared with the model group, the levels of IL-1β in the pretreatment group and the treatment group were statistically significant(P<0.01).[Conclusion] In the infection stage(3 d), the use of Hongzhongxiao and Mupirocin ointment treatment of rat skin abscess did not play to "eliminate evil" role. In the abscess formation stage(7 d), compared with Mupirocin ointment,hongzhong elimination tincture can promote rat plasma IL-10 expression, inhibition of IL-1β expression, inhibition of inflammation and promote the formation of huchang. |
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