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| Determination of Terrestrinin A from Xinnao Shutong capsules by HPLC-ELSD method |
| Hits 1651 Download times 1197 Received:January 20, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.02.14 |
| Key Words
Xinnao Shutong capsules;Tribulus terrestris L.;Terrestrinin A;HPLC-ELSD;quantitative determination |
| Author Name | Affiliation | E-mail | | ZHANG Na | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biomedicine, Research and Development Center of Traditional Chinese Drug, Tianjin 300457, China | | | LUAN Xiao-ying | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | XIE Yan | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biomedicine, Research and Development Center of Traditional Chinese Drug, Tianjin 300457, China | | | BAI Zi-song | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Tianjin International Joint Academy of Biomedicine, Research and Development Center of Traditional Chinese Drug, Tianjin 300457, China | | | BAI Yang | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | HU Li-min | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | WANG Meng | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | ZHANG Peng | Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | zhp8270@sina.com.cn |
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| Abstract
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| [Objective] To establish a quantitative method for the determination of Terrestrinin A in Xinnao Shutong capsules and total saponins. [Methods] Using HPLC ELSD method, chromatography column Hedra-C18 column (4.6 mm×150 mm, 5 microns), mobile phase:acetonitrile-water, flow rate:1 mL/min, column temperature:40℃, drift tube temperature:60℃, gas pressure (N2):30 psi, gain:100, sample quantity:20 μL. [Results] Linear range:48.5 to 970.6 μg/mL, the regression equation:Ŷ=1.693 1+1.264 4X (r2=0.9990), precision, repeatability and stability testing of RSD value was 1.21%, 1.62%, 1.9% respectively, the recovery rate is 96.0%-103.2%, RSD value was 2.4%. [Conclusion] The method is simple and reliable, and can be applied to determine the content of Terrestrinin A in Xinnao Shutong capsule and total saponins preserving method and establish quality control standards. |
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