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| Optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity study |
| Hits 1435 Download times 1290 Received:October 30, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2017.02.15 |
| Key Words
mulberry polysaccharide extacrion process;anti-aging activity;research and analysis |
| Author Name | Affiliation | E-mail | | ZHANG Pei-li | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | CHEN Xue-xiang | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | WANG Qun | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | LIU Guo | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | ZHANG Yong | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | YANG Yi | College of Food Science, South China Agricultural University, Guangzhou 510642, China | | | CAO Yong | College of Food Science, South China Agricultural University, Guangzhou 510642, China | caoyong2181@scan.edu.cn |
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| Abstract
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| [Objective] To study the optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity. [Methods] Usingel egans model, such as life, movement analysis, frequency of swallowing, spawning and intestinal brown quality comprehensive evaluation of different dose of T3-3 to study the anti-aging activity of itspurified components T3-3. [Results] The best extracted condition was:100℃ of extraction temperate, 2 h of extraction time, 3 times, and 1:30 of liquid ratio, the extracted rate would up to 8.48%. Anti-aging activity:T3-3 extend the average life span for 10.38%(P<0.05), the corresponded time of 50% survival rate extend 0.91%; the deglutition frequency is 18% higher than the control group; the egg laying amount have no significant difference; the intestinal lipofusin produced by nematode is reduced, 11.14%(P<0.05) lower than the fluorescence intensity of control group. At the final stage of experiment, the average fluorescence intensity of control group increase 12.47%.[Conclusion] The results provide that the concentration of 150 mg/L, T3-3 has the best effect. |
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