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| Schizandrin B alleviate bleomyci-induced pulmonary fibrosis |
| Hits 1765 Download times 1393 Received:February 14, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.03.11 |
| Key Words
Schizandrin B;pulmonary fibrosis;anti-oxidation;TGF-β1;p-Smad2 |
| Author Name | Affiliation | E-mail | | WEI Fei | Tianjin University of Traditional Chinese Medicine, Tianjin, 300193
Hospital Logistics College Chinese People's Armed Police Force, Tianjin 300162, China | | | LIU Bin | Hospital Logistics College Chinese People's Armed Police Force, Tianjin 300162, China | | | XIAO Na | Beijing Nanyuan Hospital, Beijing 100076, China | | | CAO Bo | Logistics University of People's Armed Police Force, Tianjin 300309, China | | | WEI Lu-qing | Hospital Logistics College Chinese People's Armed Police Force, Tianjin 300162, China | luqing-wei@163.com |
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| Abstract
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| [Objective] To discuss the effect and mechanism of Schizandrin B in the process of pulmonary fibrosis in mice.[Methods] A total of 96 mice were randomly divided into four groups (n=24) and pulmonary fibrosis models were established using the bleomycin solution injected through trachea of the mouse in M,DM,Sch-B groups except the mouse in control group.After the models were established,the mouse in N and M groups were given olive oil,the mouse in DM were given dexamethasone and Sch-B groups were given Schizandrin B respectively.The mice were sacrificed at day 7,14,28 after administration.HE and Masson staining were performed to observe the pathological changes of lung tissues.The content of IL-6 in serum were detected by ELISA.The levels of SOD,GSH and HYP were measured.The expressions of TGF-β1,p-Smad2 and α-SMA in lung tissue were detected by immunohistochemical method to value treatment effect.[Results] The alveolitis and fibrosis extent significantly reduced in the group were treated by Schizandrin B.Compared with model group,inflammatory biomarkers and antioxidant indices were obviously rebounded in Schizandrin B group.Protein express of TGF-β1,p-Smad2 and α-SMA in Schizandrin B group reduced significantly as compared with that of model group.[Conclusion] Schizandrin B can alleviate bleomycin-induced pulmonary fibrosis in mouse.Inhibiting inflammatory response,elevating antioxidant capacity and regulating TGF-β1,p-Smad2,α-SMA may be part of mechansim. |
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