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| HPLC for simultaneous determination of various quota components in Yunvjian |
| Hits 1611 Download times 1455 Received:March 05, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.03.14 |
| Key Words
Yunvjian;gradient elute;simultaneoud determination;HPLC;mangiferin;β-ecdysterone;verbascoside |
| Author Name | Affiliation | E-mail | | WANG Ting-ting | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | WANG Ya-jing | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | yajing022@163.com | | SUN Ai-zhen | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | ZHOU Hao | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | ZHU Guang-mei | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | LI Na | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | ZHANG Zhe | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | TIAN Qian-yu | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | |
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| Abstract
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| [Objection] To establish an HPLC method for simultaneous determina-tion of mangiferin,β-ecdysterone,verbascoside in Yunvjian.[Methods] The HPLC separetion was performed on Ultimate XB-C18 column (4.6 mm×250 mm,5 μm) eluted with the mobile phase as acetonitrile-0.1% phosporic acid solution in a gradient mode at a flow rate of 1.0 mL/min.The detection wavelength was 250 nm for mangiferin and β-ecdysterone,and 330 nm for verbascoside.[Results] The calibration curve was liner within the range of 10.0-400.0 μg/mL (r=0.999 9) for mangiferin,2.4~96.0 μg/mL (r=0.999 9) for β-ecdysterone,and 2.0~80.0 μg/mL (r=0.999 9) for verbascoside,and the average recovery were 100.2%,101.0%,98.1 8% with RSD were 2.73%,1.95% and 1.39%(n=9).[Conclusion] The method is simple and accurate,and can be used for the quality control of Yunvjian. |
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