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| Effect of the expression of CD40 signal in endothelial cells with ox-LDL and anti-inflammatory effect of Astragaloside IV |
| Hits 1613 Download times 1397 Received:March 31, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.04.12 |
| Key Words
endothelial cells;ox-LDL;CD40;anti-Inflammatory;atherosclerosis |
| Author Name | Affiliation | E-mail | | WEI Bing | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | JIANG Xi-juan | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | LU Bin | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | SUN Ying-xin | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | LI Hu-hu | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | ZHANG Tong | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | LI You-you | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | | | GUO Mao-juan | Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China | 39273229@qq.com |
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| Abstract
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| [Objective] Effect of the expression of CD40 signaling in endothelial cells (ECV-304) with modified low density lipoprotein (ox-LDL) and anti-inflammatory effects of Asragaloside IV.[Methods] ECV-304 with 100 mg/L ox-LDL for 24 h, then measured the expression of CD40 mRNA and protein by Real-time PCR and Western blot. The content of TNF, sVCAM-1, IL-6 and IL-8 in cell supernatant were determined by ELISA assay. With anti-CD40 antibody was added to ECV-304, and so did the same of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant. ECV- 304 with 20 g/mL Astragaloside IV preincubation for 4 h, and then with 100 mg/L ox-LDL for another 24 h, checking the CD40 mRNA and protein and the content of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant.[Results] The expression of CD40 mRNA protein were up-regulated with Ox-LDL in ECV-304, and promoted cell secretion of TNF-α, sVCAM-1, IL-6 and IL-8.However, the protein of CD40 were inhibited, and TNF-α, sVCAM-1, IL-6 and IL-8 were all down- regulated. Astragaloside IV could down regulate the expression of CD40 mRNA and protein, and inhibit the secretion of TNF-α, sVCAM-1, IL-6 and IL-8.[Conclusion] Astragaloside IV could inhibit the expression of ox-LDL induced inflammatory mediators in endothelial cells by inhibiting CD40 signaling, which is a potential mechanism of anti-atherosclerosis. |
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