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| Isolation, culture and purification of human endometrial glandular epithelial cells in vitro |
| Hits 1694 Download times 1637 Received:March 16, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.04.14 |
| Key Words
endometriosis;endometrial glandular epithelial cells;cells culture |
| Author Name | Affiliation | E-mail | | WANG Yue-ling | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Pei-lin | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | lipeilin@wo.cn | | LI Jie | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | WANG Jian-ling | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | HUA Dan-dan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Qian-nan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Cui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] Improvement the isolation, culture and purification technology of human endometrial glandular epithelial cells to obtain lots of the economic cells with good activity and high purity, and provide reliable model in vitro to study of endometriosis.[Methods] Repeated digestion of composite enzyme, single filtration of screen mesh with 220 μm in diameter, low speed centrifugal and differences in time of adherent cells.[Results] The human endometrial glandular epithelial cells were successfully cultured in 18 of 19 endometrial tissues, which were obtained from the method of diagnostic curettage. The putity of human endometrial glandular epithelial cells was up to 94.2%.[Conclusion] By the methods of repeated digestion of composite enzyme, single filtration of screen mesh with 220 μm in diameter, low speed centrifugal and differences in time of adherent cells, we can obtain lots of the economic cells with good activity and high purity. |
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