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| Determination of four amino acids in Shuxuening injection by HPLC coupled with precolumn derivatization |
| Hits 1789 Download times 1406 Received:April 23, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.04.15 |
| Key Words
Shuxuening injection;precolumn derivatization;aspartic acid;threonine;alanine;methionine |
| Author Name | Affiliation | E-mail | | CHEN Ying | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China | | | CAO Jun | College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China | | | GUAN Xiu-wei | Hebei Shineway Pharmaceutical Co., Ltd., Langfang 065200, China | | | ZHANG Gang | Hebei Province Engineering Research Center for TCM Injection, Langfang 065200, China | | | LI Zhi-gang | Chinese medicine injection new drug development technology national local joint engineering laboratory, Langfang 065200, China | lizhigang@shineway.com | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To establish a HPLC method for determining 4 amino acids (aspartate, threonine, alanine and methionine) in Shuxuening injection.[Methods] HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent. Aspartate, threonine, alanine and methionine in Shuxuening injection were analyzed on C18 column with gradient elution of acetonitrile-water (5:1) and sodium acetate buffer solution (pH=6.5). The detection wavelength was at 254 nm.[Results] Separation and the linear relationships of 4 amino acids were good. All RSDs of precision, repeatability and stability were less than 5%. The sample recoveries were between 95% and 98%, with RSD less than 5%. The total content of four amino acids in Shuxuening injection was 1 738.47 μg/mL. The content of methionine was the highest in Shuxuening injection, while the content of alanine was the lowest.[Conclusion] The accurate and simple method was reliably applied to determine content of four amino acids in Shuxuening injection which could provide a scientific tool for the quality control of Shuxuening injection. |
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