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| Rat intestinal absorption study of Zexie decoction with Ussing chamber by UPLC-MS/MS |
| Hits 1303 Download times 1321 Received:April 10, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.04.16 |
| Key Words
Zexie decoction;Ussing chamber;LC-MS/MS;Atractylenolide Ⅱ;Atractylenolide Ⅲ |
| Author Name | Affiliation | E-mail | | ZHU Yun | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | GU Xing | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | ZHANG Bing | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | QI Dong-li | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | qidongli2013@163.com | | LIU Zhi-dong | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | WEI Zi-bin | Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | |
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| Abstract
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| [Objective] To investigate ratintestinal absorption of Atractylenolide Ⅱ and Atractylenolide Ⅲ in Zexie decoction.[Methods] The column was Agilent Zorbax Plus C18 (4.6 mm×12.5 mm, 5 μm). The mobile phase consisted of a mixture acetonitrile-0.1% formic acid aqueous solution (42:58), the flow rate was 0.3 mL/min. Quantification was performed in the positive ionization by MRM mode. The monitored transitions were set at m/z 233.2→m/z187 and m/z 249.15→m/z231for Atractylenolide Ⅱ and Atractylenolide Ⅲ, respectively.[Results] The apparent permeability coefficients (Papp) of Atractylenolide Ⅱ was gradually on the increase in duodenum, jejunum, ileum and colon. The same thing happened to the Papp of Atractylenolidein Ⅲ in jejunum, duodenum, ileum and colon.[Conclusion] This method was simple, precise and repeatable, which could be used for intestinal investigation of Zexie decoction. |
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