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| Degradation kinetics of four active ingredients in compound of Xuezhining |
| Hits 1761 Download times 1405 Received:April 15, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.05.12 |
| Key Words
compound of Xuezhining;pH;temperature;degradation kinetics |
| Author Name | Affiliation | E-mail | | LIU Han | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | REN Xiao-liang | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | xiaoliang_ren@sina.com | | ZHANG Hui-jie | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SUN Li-li | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Meng | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To study the degradation kinetics of four main active ingredients in compound of Xuezhining under different pH values and temperatures.[Method] HPLC method was established to study the stability ofRubrofusarin-6-O-β-gentiobioside(HLMS), 2, 3, 5, 4-tetrahy-droxystilbene-2-O-β-D-glycoside(THSG), Nuciferine (HYJ) and Hyperoside (JSTG),four active ingredients in compound of Xuezhining, under different pH values (1.2, 6.8 and 8.3) and different temperatures (60, 70, 80, 90℃). Degradation kinetic parameters were evaluated by chemical kinetics.[Results] The results indicated that HLMS, THSG, HYJ and JSTG were degraded at different degree under different pH values and temperatures, and accord with the first-order kinetic. Temperature has great effect on the degradation rate, and the degradation rate increased significantly with the increase of temperature. According to arrhenius equation, activation energy (Ea) of HLMS, THSG, HYJ and JSTG in aqueous solution were 90.56 kJ/mol,74.66 kJ/mol, 72.66 kJ/mol and 74.42 kJ/mol, respectively.[Conclusion] The four active ingredients in compound of Xuezhining were sensitive to high temperature condition. At the condition of room temperature, HYJ and HLMS were slower in different pH buffer solution, while JSTG and THSG degraded faster in weak alkalinity condition. |
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