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| Effect of four chemical components dissolution in Siwu decoction by decocting together and single |
| Hits 2001 Download times 1443 Received:May 10, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.05.14 |
| Key Words
Siwu decoction;mixed-decoction;single-decoction;peoniflorin;ferulic acid;ligustilide;senkyunolide I |
| Author Name | Affiliation | E-mail | | GUO Jiang-yu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SUN Lei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YAN Yan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU He-shui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hsyu@tjutcm.edu.cn | | LI Wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Li-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SONG Xin-bo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | songxinbo@tjutcm.edu.cn |
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| Abstract
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| [Objective] To establish a method for content determination of peoniflorin, ferulic acid, ligustilide and senkyunolide I in Siwu decoction and study the dissolution variation between mixed-decoction and single-decoction.[Methods] RP-HPLC with Cosmosil C18 (4.6 mm×250 mm, 5 μm) was used, methanol-0.3% formic acid solution gradient elution was applied. The flow rate was 1.0 mL/min. The detection wavelength was 280 nm and the column temperature was 30℃. The dissolution variation was analyzed between mixed-decoction and single-decoction.[Results] The linear range of four components were 19.38~310.00 μg/mL (r=0.999 4), 1.51~24.16 μg/mL (r=0.999 4), 1.50~24.00 μg/mL (r=0.999 6) and 2.00~32.00 μg/mL (r=0.999 4) with average recovery 95.89%(RSD=2.90%), 97.08%(RSD=2.28%), 96.84%(RSD=2.87%) and 98.77%(RSD=1.98%) respectively. The dissolution of senkyunolide I was lower mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of ferulic acid and ligustilide were higher mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of peoniflorin was higher mixed-decoction than single-decoction of Radix paeoniae Alba.[Conclusion] The dissolution of chemical compositions were not a simple superposition of their dissolution in single medicine, but have their own inherent compatibility regularity. This research from the perspective of the chemical composition dissolution provides a reference for the study of Siwu decoction. |
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