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| Yizhi Ningshen granules:Effects on Dopamine levels, GDNFmRNA and its protein expression in prefrontal cortex-striatum of SHR |
| Hits 3271 Download times 1287 Received:July 20, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2017.06.13 |
| Key Words
Yizhi Ningshen granules;attention deficit hyperactivity disorder;dopamine;glial cell line-derived neurotrophic factor;SHR rat |
| Author Name | Affiliation | E-mail | | LI Rui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Ya-ping | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | zf_ts@163.com | | MA Rong | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Wei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHONG Cheng-liang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WEI Xiao-wei | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] In order to explain the mechanism of Chinese medicine compound granules, which named Yizhi Ningshen as the treatment of ADHD more than ten years, this study aimed to study the effects of Yizhi Ningshen granules on dopamine content, GDNFmRNA and protein expression in prefrontal and striatum of young SHR rats combined with modern molecular biology techniques.[Methods] Thirty-two young SHR rats were randomly divided into model group, Yizhi Ningshen group, methylphenidate hydrochloride group and atomoxetine group, 8 WKY rats were randomly divided into normal control group. The rats were sacrificed for 4 weeks of continuous administration using designed doses, and the contents of dopamine in the prefrontal and striatum were detected by HPLC and the expression of GDNF mRNA was detected by RT-PCR, the expression of GDNF protein was detected by western blot.[Results] 1)The content of DA in the prefrontal cortex and striatum of SHR group was lower than that of WKY group, the content of DA in the prefrontal cortex was significantly higher than that in the model group (P<0.05). The content of DA in the striatum of the Yizhi Ningshen group was lower than that in the normal control group and tomoxetine group (P<0.05). 2)The expression of GDNF mRNA in the prefrontal cortex and striatum of SHR group from high to low order, the sequence is as follows, tomooxetine group > methylenidate group > Yizhi Ningshen group > model group (P<0.05). 3)The expression of GDNF protein in the prefrontal cortex and striatum of each SHR group was higher than that in the WKY group, while the expression in striatu was lower than the methylphenidate group (P<0.05).[Conclusion] Yizhi Ningshen granules can increase DA content and its specific nutrition factor GDNF mRNA and protein expression in specific brain regions of young SHR. It may play a role in promoting the maturation of dopaminergic neurons in specific brain regions of young SHR rats. |
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