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| Effect of Qihuang granule on experimental choroidal neovascularization VEGF Protein and its mRNA expression |
| Hits 1356 Download times 1226 Received:August 30, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.01.13 |
| Key Words
Qihuang granule;VEGF;CNV;WAMD |
| Author Name | Affiliation | E-mail | | QUAN Ying | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIANG Fengming | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | liangfm66@163.com | | WANG Li | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yan | Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510000, China | | | LI Hongmei | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUAN Yushuang | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yingying | First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To investigate the effect of Qihuang granule on expression of vascular endothelial growth factor protein and its mRNA in laser-induced choroidal neovascularization.[Methods] Eighty-one male brown Norway rats were randomly divided into two groups:13 for the blank control group; 68 were irradiated with 532 laser light. Three rats among the irradiated rats were randomly selected to the form of cnv and others were randomly divided into 5 groups:Qihuang granule group A (Gavage 60 days), Qihuang granule group B (Gavage 90 days), lucentis group, Qihuang granule combine lucentis group and the model group,with different drugs and time of treatment respectively. Expect Qihuang granule group B rats gavaged for 90 days, Others with 60 days, all retinal pigment epithelium-choroid-sclera complex form were geted to detect the protein content and mRNA expression of VEGF.[Results] After modeled 7 days, suggestting CNV formated. after 60 days intervention, VEGF protein content change, Qihuang granule combine lucentis group compared with Qihuang granule group A,Qihuang granule group A combine lucentis group compared with the the model group,VEGF protein content was reduced, with statistical differences(P<0.05). Qihuang granule group B compared with Qihuang granule group A, VEGF protein content were decreased, with statistically significant after 60 days intervention(P<0.05), VEGF mRNA change:compared the blank group with the model group, Qihuang granule combine lucentis group compared with the model group, VEGF mRNA were decreased with statistical differences(P<0.05). Qihuang granule group B compared with Qihuang granule group A,VEGF mRNA were decreased with statistically significant(P<0.05).[Conclusion] The method of Qihuang granule combine lucentis, can regulate the expression of VEGF, reduce the formation of CNV, and has a certain therapeutic significance to wet age-related macular degeneration. |
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