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| Construction and proprieties of a puerarin-interact, injectable and Ca2+ responsive gel |
| Hits 1716 Download times 1332 Received:August 29, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.01.15 |
| Key Words
puerarin;calcium alginate;injectable gel;ion responsive gel;osteoarthritis |
| Author Name | Affiliation | E-mail | | YUE Zhanlong | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CUI Yuanlu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | cuiy@tju.edu.cn |
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| Abstract
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| [Objective] A puerarin-interact calcium alginate scaffold gel was build for the treatment of osteoarthritis. The gel has the properties of injectable, Ca2+-responsive, controlled release of drugs as well as the Mesenchymal Stem Cells (MSCs) support characteristic.[Methods] Sodium alginate (SA), CaCl2 and puerarin (Pu) were used as the raw materials for the preparation of gels. Explore the effects of Pu on viscosity by the Viscometer. The best formulation was chosen by the method of Rheology and the effects of Pu on gelatinization were explored. The microstructure of lyophilized gels was observed. Their properties of welling ratio, vitro degradation and drug release were studied.[Results] The homogeneous and transparent gels were obtained by physical stirring. The effect of raw materials on the gel strength was in order of CaCl2 > SA > Pu and the best formulation was 0.2% Pu+0.6% SA+6 mmol/L CaCl2, Their 3D mesh structures were showed by the SEM. The characterization experiments suggest that Pu has promoting effects on gel strength. Compared with the Ca2+-alginate gel, the swelling ratio of Pu-calcium alginate gel decrease from 75 to 45 times and the initial degradation time prolonged from 12 d to 24 d. The gel showed sustained-release property on alkaloid Tetrandrine.[Conclusion] Pu was involved in the construction of calcium alginate gel, which increased the gel strength and improved the properties of swelling and vitro degradation. The gel had better strength and showed 3D mesh structure. It had sustained-release property on alkaloid Tetrandrine. The Pu-calcium alginate gel was expected to be the injectable scaffolds of drugs or MSCs for the treatment of osteoarthritis. |
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