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| Simultaneous determination of six components such as mangiferin in Dayuan yin by HPLC |
| Hits 1672 Download times 1110 Received:November 13, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.01.17 |
| Key Words
Dayuan yin;determination of contents;mangiferin;paeoniflorin;baicalin |
| Author Name | Affiliation | E-mail | | WANG Jinyan | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | REN Jing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | CHEN Shibin | China Resources of Sanjiu Medical & Pharmaceutical CO., LTD., Shenzhen 518110, China | | | YAO Shaozi | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | MA Li | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | LIU Zhidong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] Use HPLC to establish a method which can determine 6 kinds of index component in Dayuan yin, such as mangiferin, paeoniflorin, baicalin, glycyrrhizic acid, magnolol and honokiol.[Methods] The Agilent Zorbax Eclipse Plus C18 (4.6 mm×250 mm, 5 μm) column was adopted; the mobile phase was acetonitrile (A)-0.2% aqueous phosphoric acid (B) by a gradient eluted program:0~40 min, 10%~40% A; 40~45 min, 40%~70% A; 45~50 min, 70% A; 50~55 min, 70%~10% A; 55~60 min, 10% A. The flow rate was 0.8 mL/min, The column temperature was 30℃, The detection wavelength was 230 nm.[Results] The separation degree of the 6 components was good, and all the components had good linear relationship in the linear range, and the recoveries (n=9) were within the range of 95.0%~103.0%, the RSD% were less than 3.0%.[Conclusion] The method is simple, accurate, sensitive and reproducible, and can used to determine the contents of Dayuan yin. |
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