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| Effect of Danggui Buxue decoction on the expression of GRP78-PERK-eIF2α in diabetic nephropathy rats |
| Hits 1592 Download times 1257 Received:October 21, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.02.11 |
| Key Words
Danggui Buxue decoction;endoplasmic reticulum stress;PERK pathway;diabetic nephropathy |
| Author Name | Affiliation | E-mail | | SHEN Xin | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | ZHANG Siquan | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | ZHANG Yingwen | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | hhao3838@sina.com | | KE Haoliang | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | SHUAI Yu | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | |
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| Abstract
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| [Objective] To observe the effects of Danggui Buxue decoction on the expression of glucose-regulated protein 78(GRP78), protein kinase R-like ER kinase (PERK), eukaryotic translation initiation factor 2 alpha(eIF2α) in diabetic nephropathy rats and explore the mechanism of its effect on the prevention and treatment of DN.[Methods] Fifty rats were randomly divided into blank group(N), model group, gliclazide gloup, low and high dose of Dangguibuxue decoction (DL/DH). The rat model of DN was established by intraperitoneal injection of streptozotocin (STZ) combined with high fat diet. After 8 weeks, the changes of renal structure were observed under light microscope and electron microscope;the serum glucose,lipids and the renal function related parameters were detected by enzyme coupling colorimetric method; the expression of GRP78, PERK and eIF2α protein in renal tissue were detected by immunohistochemistry and western blot and the mRNA expression of GRP78, PERK and eIF2α was detected by Real-time PCR.[Results] The rats in the DN group were in poor condition, the renal function and the kidney structure were seriously damaged, the expression of GRP78, PERK and eIF2α protein and mRNA in the DN group were significantly worse than those in the N group, the expression of GRP78,PERK and eIF2α protein and mRNA decreased significantly after treatment, the DH group decreased more significantly in a concentration-dependent manner.[Conclusion] Danggui Buxue decoction can inhibit the expression of GRP78, PERK and eIF2α in DN rats kidney, meanwhile, it can relieve endoplasmic reticulum stress and protect renal function. |
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