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| Effect of Huangdan Huayu decoction on cerebral ischemia reperfusion injury and expression of BDNF and VEGF in brain tissue of rats |
| Hits 1635 Download times 1216 Received:December 26, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.02.13 |
| Key Words
Huangdan Huayu decoction;cerebral ischemia reperfusion;BDNF;VEGF;VEGFR-1 |
| Author Name | Affiliation | E-mail | | LU Lifu | Department of pharmacy, Puyang City Hospital of traditional Chinese Medicine, Puyang 457000, China | | | QIU Xueyin | Department of oncology Puyang People's Hospital, Puyang 457000, China | xcx13839293129@126.com |
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| Abstract
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| [Objective] To investigate the effect of Huangdan Huayu decoction on cerebral ischemia reperfusion injury in rat brain derived neural brain tissue growth factor (BDNF), vascular endothelial growth factor (VEGF) expression.[Methods] A total of 60 SD rats (male)were randomly divided into 5 groups (There were 12 rats in each group):the sham operation group, model group, low dose group[7.20 g/(kg·d)], middle dose group[14.40 g/(kg·d)], high dose group[28.80 g/(kg·d)]. A cerebral ischemia reperfusion model was prepared by wire suture method, reperfusion was performed after ischemia 2 h, 24 h rats were sacrificed after reperfusion. Neurobehavioral score (zealonga score) was performed,the expression of BDNF, VEGF and VEGFR-1 protein was detected byimmunohistochemistry and Western blot, the expressions of BDNF, VEGF and VEGFR-1 in each group were observed.[Results] The cerebral infarction volume and neurological function score of model group after cerebral ischemia reperfusion 24 h were compared with those of sham operation group,the difference was statistically significant(P<0.05); the middle dose group, high dose group, cerebral infarction volume and neurological function were compared with the model group, the difference was statistically significant (P<0.05); the expression of VEGF, BDNF and VEGFR-1 in the model group was significantly higher than that in the sham operation group, and the difference was statistically significant (P<0.05); the middle dose group, high dose group VEGF, BDNF, VEGFR-1 protein expression was significantly higher than the model group, the difference was statistically significant (P<0.05); the small dose group was compared with the model group,the expression of VEGF, BDNF, VEGFR-1 protein in the small dose group was not statistically significant (P>0.05).[Conclusion] Huangdan Huayu Decoction reperfusion cerebral injury rats are treated for cerebral ischemia,it can exert neuroprotective effect by up regulating the levels of VEGF, BDNF and VEGFR-1 protein. |
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