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| Preparation and preliminary evaluation of compound plantaginis semen gel paste |
| Hits 1806 Download times 1567 Received:November 20, 2017 |
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| DOI
10.11656/j.issn.1673-9043.2018.02.15 |
| Key Words
Plackett-Burman;Box-Behnken;Gel Paste;in vitro release;H22 hepatoma ascites model |
| Author Name | Affiliation | E-mail | | XIA Zhenzhen | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | CHEN Cheng | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LI Bingshao | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | 王永洁 | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Yongjie | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LIN Hongmei | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | WU Qing | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | qwu@vip.sina.com |
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| Abstract
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| [Objective] The aim of this study is to prepare compound Plantaginis semen Extractum(CPE) as an easy-to-use Compound Plantaginis semen Gel Paste(CPGP).[Methods] Plackett-Burman design combined with Box-Behnken esponse surface methodology was used to optimize the preparation formulation with initial bonding strength, holding force, peel strength of 180° as the evaluation index. Modified Franz diffusion cell was used to study in vitro release behavior of CPGP. A preliminary evaluation of the efficacy of CPE and CPGP on the H22 hepatoma ascites model in mice was carried out.[Results] The optimized matrix formulation of CPGP is NP700, PVPK90, glycerol, aluminium glycinate, tartaric acid, distilled water (8:5.38:32.5:0.5:0.5:44.62). The in vitro release rate of geniposide in the CPGP was (81.23±1.52)% at 24 h. The results showed that the CPGP formulation was reasonable. The viscosity of gel paste prepared by the formulation was suitable. In animal experiment, compared with the model group, the volume of ascites mice grouped in CPGP and CPE was very significantly decreased (P<0.01) and the VEGF content in ascites was significantly decreased (P<0.05), also the destruction of immune function was significantly improved (P<0.05) after the treatment for 7 days. Meanwhile the gel paste group and extract group showed no significant difference.[Conclusion] Therefore, the research showed a reasonable CPGP prescription. The release rate of the target component geniposide was in accord with the first order kinetic equation. CPGP effectively reduced the amount of ascites in mice. |
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