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| Inhibitory effects of total saponins of tupistra chinensis in human medullary thyroid carcinoma TT cells and its effect on expression of Notch1 |
| Hits 1396 Download times 1233 Received:January 04, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2018.03.13 |
| Key Words
Tupistra chinensis Baker;medullary thyroid carcinoma;Notch1;DAPT |
| Author Name | Affiliation | E-mail | | SHEN Xin | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | XIA Yukun | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | ZHANG Yingwen | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | hhao3838@sina.com | | ZHOU Zhen | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | AI Wang | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | | | ZHANG Peng | Zhongnan Hospital of Wuhan University, Wuhan 430071, China | |
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| Abstract
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| [Objective] To study the inhibitory effects of total saponins of Tupistra chinensis Baker (TST) in human medullary thyroid carcinoma TT (MTC-TT) cells and its effect on expression of Notch1 and investigate its anticancer mechanism.[Methods] MTC-TT cells were treated with different density of TST, the cell proliferation inhibition rate and IC50 at 24 h, 48 h, 72 h were evaluated by CCK8;the disposition of MTC-TT cells cycle was detected by flow cytometry. The expression of Notch1 protein were detected by western blot and the mRNA were detected by Real-time PCR; in addition, the expression of Notch1 protein were detected by western blot and the cell proliferation inhibition rate was detected by CCK8 after TST and DAPT (Notch1 inhibitor) treated 48h.[Results] The IC50 at 24 h, 48 h,72h was 59.01, 31.54 and 31.63 μg/mL respectively. Compared to control group, TST has inhibitory effects on MTC-TT cells (P<0.01), cell cycle were arrested in G2/M phase (P<0.05, P<0.01). Compared to control group,the expression of Notch1 protein and mRNA in experience group increased significantly after treatments (P<0.05, P<0.01), the high dose group increased more significantly in a concentration-dependent manner (P<0.01). Compared to TST group, the expression of Notch1 protein in TST +DAPT group decreased significantly (P<0.01), the cell proliferation inhibition rate decreased,either (P<0.01).[Conclusion] TST could inhibit the proliferation and cell cycle of MTC-TT cells by probable up-regulation of the Notch1 expression. |
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