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| The effection of Yiqi Yangyin Huoxue decoction on liver pathological changes and miRNA-126 expression level in liver of T2DM rats |
| Hits 1673 Download times 1002 Received:January 05, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2018.03.14 |
| Key Words
Yiqi Yangyin Huoxue decoction;diabetic rats;liver pathology;miRNA-126 |
| Author Name | Affiliation | E-mail | | LIU Ying | Endocrinology Department One, Tangshan City of Traditional Chinese Medicine Hospital, Tangshan 063000, China | | | ZHANG Jun | Endocrinology Department One, Tangshan City of Traditional Chinese Medicine Hospital, Tangshan 063000, China | tszhangj@126.com | | ZHAO Jing | North China University of Science and Technology, Tangshan 063000, China | | | WANG Haiying | North China University of Science and Technology, Tangshan 063000, China | | | WANG Junyuan | Endocrinology Department One, Tangshan City of Traditional Chinese Medicine Hospital, Tangshan 063000, China | | | WANG Mingxing | North China University of Science and Technology, Tangshan 063000, China | | | LIU Xiaoman | North China University of Science and Technology, Tangshan 063000, China | | | WANG Shunyi | North China University of Science and Technology, Tangshan 063000, China | | | DAI Jinying | North China University of Science and Technology, Tangshan 063000, China | | | YUAN Jie | North China University of Science and Technology, Tangshan 063000, China | |
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| Abstract
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| [Objective] To discuss the the mechanism of Yiqi Yangyin Huoxue decoction to improve insulin resistance in diabetic rats by measuring the expression level of miRNA-126 using the realtime-quantitative PCR (qRT-PCR) technology.[Methods] Sixty wistar male rats of 12 weeks old were randomly divided into two groups, including 10 blank groups and 50 rats in the experimental group.The high-fat diet plus low-dose streptozotocin (STZ) rat model of type 2 diabetes mellitus induced. After the success of the model, 50 diabetic rats were randomly divided into 5 groups, normal group 10 and model group 10 were treated with normal saline, and Jinlida group with Jinlida particles, and TCM low,medium,high dose group, treated with the corresponding dose of Chinese medicine orally, 4 weeks of treatment, blood biochemical indicators of blood glucose, glycosylated serum protein, fasting insulin levels, the liver tissue, HE staining, observation of liver tissue of rats under light microscope. Determination of liver tissue miRNA-126 expression level by RT-PCR method.[Results] Jinlida granules and traditional Chinese medicine Yiqi Yangyin Huoxue decoction (TCM) both can significantly reduce the blood sugar,and fasting insulin level of diabetic rats,and TCM medium dose is better than Jinlida granules. Pathological morphology of the Chinese medicine groups of liver cells under light microscope were significantly improved. Both Jinlida granules and TCM can decrease the content of miRNA-126 in liver tissues, with a better effect of the medium dose of TCM.[Conclution] Yiqi Yangyin Huoxue recipe can improve the blood glucose metabolism disorder in type 2 diabetic rats, and improve the pathological damage of liver, which may be related to the reduction of the expression level of miRNA-126 in the liver. |
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