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| Study on the mechanism of Hydroxy safflower yellow pigment in bleomycin induced interstitial lung disease in mice |
| Hits 1524 Download times 1084 Received:April 15, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2018.05.11 |
| Key Words
Hydroxy safflower yellow pigment;interstitial lung disease;TGF-β1;IL-6;PAI-1;VEGF mRNA |
| Author Name | Affiliation | E-mail | | WANG Ying | Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | | SUN Yupeng | Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | | ZHANG Wei | Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China | huxizhijia@126.com |
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| Abstract
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| [Objective] To study the mechanism of Hydroxy safflower yellow pigment in bleomycin induced interstitial lung disease in mice.[Methods] A total 90 Balb/c mice were divided into 3 groups, that is control group, model group group and Hydroxy safflower yellow pigment group. After 21days, 3 mice were dissected at random,and After 28 days,all mice were dissected and lung tissues were taken,while the experiment was finished. Masson staining can show the degree of inflammatory cell infiltration in mice lung tissues; Enzyme-linked immunosorbent assay was used to detect the expression levels of TGF-β1, IL-6; RT-PCR assay was applied to detect the expression levels of PAI-1and VEGF in protein levels.[Results] The expression of TGF-β1、VEGF mRNA in model group were higher significantly than control group(P<0.01), while these indexes of Hydroxy safflower yellow pigment group lower significantly thanmodel group(P<0.01). The expression of IL-6 in model group were higher significantly than control group(P<0.01), while this indexes of Hydroxy safflower yellow pigment group lower thanmodel group(P<0.05). The expression of PAI-1 mRNA in model group were higher significantly than control group(P<0.01), while this indexes of Hydroxy safflower yellow pigment group was not significantly correlated.[Conclusion] Hydroxy safflower yellow pigment can reduce the expression of TGF-β1, IL-6, VEGF mRNA, and prevent or reverse pulmonary fibrosis. |
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