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| Determination of 3 atractylenolides in Atractylodes macrocephala Koidz. and the study of the fingerprint |
| Hits 1779 Download times 1319 Received:May 22, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2018.05.15 |
| Key Words
Atractylodes macrocephala Koidz.;atractylenolide;content determination;fingerprint;hierarchical cluster analysis |
| Author Name | Affiliation | E-mail | | ZHANG Bing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | ZHANG Lina | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | HAN Pengjun | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | HUANG Rui | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | QI Dongli | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | qidongli2013@163.com | | LIU Zhidong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To control the quality of Atractylodes macrocephala Koidz. from different provinces by determining the contents of AtractylenolideⅠ, Ⅱ, Ⅲ, establishing the fingerprint and clustering analysis.[Methods] The contents of AtractylenolideⅠ, Ⅱ, Ⅲ were determined on a HPLC-UV system at two wavelengthes and the fingerprint was established at a single wavelength. Twelve batches of Atractylodes macrocephala Koidz. from four different provinces were analyzed.[Results] The quantitative evaluation of AtractylenolideⅠ, Ⅱ, Ⅲ had good linear range, and the recovery were qualified. The content of AtractylenolideⅠ, Ⅱ, Ⅲ from different producing regions varied between 1.9~3.8 times. The content of atractylenolides in Atractylodes macrocephala Koidz. from Zhejiang was 2.5 times as the content of material from Anhui. The fingerprint of Atractylodes macrocephala Koidz. from different producting regions was highly similar. The similarity varied between 0.972~0.998. The results of hierarchical cluster analysis have good consistency with content determination and fingerprint analysis.[Conclusions] The characteristic method for the determining Atractylenolide Ⅰ, Ⅱ, Ⅲ and the fingerprint method based on holistic view combining with hierarchical cluster analysis were better to control the quality of the Atractylodes macrocephala Koidz. |
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