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| Simultaneous Determination of Eight Active Ingredients in Niuhuang Qinggan Capsules by HPLC Method |
| Hits 1670 Download times 953 Received:October 23, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2019.01.16 |
| Key Words
Niuhuang Qinggan capsule;HPLC;cryptochlorogenic acid;baicalein;forsythiaside A |
| Author Name | Affiliation | E-mail | | ZHUANG Yan | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | DI Tiejun | Heilongjiang Aolidaned Pharmaceutical Co., Ltd, Harbin 150031, China | | | HUO Jinhai | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | ZHU Ligang | Heilongjiang Aolidaned Pharmaceutical Co., Ltd, Harbin 150031, China | | | ZHAO Xueying | Heilongjiang University Of Chinese Medicine, Harbin 150040, China | | | ZHOU Youcai | Heilongjiang Aolidaned Pharmaceutical Co., Ltd, Harbin 150031, China | aldpd@126.com | | WANG Weiming | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | zyyjy@163.com |
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| Abstract
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| [Objective] To establish HPLC determination of Niuhuang Qinggan capsule for quality control of the preparation.[Methods] Waters Alliance 2695 HPLC is used on a C18 column (4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-0.5% phosphoric acid solution. Elution with gradient,column temperature is 40°,the flow rate is 1.0 mL/min,detection wavelength is 350 nm.[Reslts] A method was established for the simultaneous determination of cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid C,forsythiaside A,baicalin,baicalein,wogonin and wogonin in 8 components of Niuhuang Qinggan Capsules.[Conclusion] This method is stable,accurate,reproducible,and highly specific. It can be used for the quality control of Niu Huang Qinggan capsules. |
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