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| Study on the method of multi-component quality control before and after processing of corydalis yanhusuo |
| Hits 1450 Download times 1117 Received:October 29, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2019.01.17 |
| Key Words
Corydalis yanhusuo;UPLC;alkaloid;content;process |
| Author Name | Affiliation | E-mail | | SONG Hongwei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MAO Rui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Lihong | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Piao | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DOU Zhiying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | zhiyingdou@163.com | | MA Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To establish an UPLC method for the simultaneous determination of eightalkaloids(tetrahydrocolumbamine,protopine,tetrahydrocoptisine,columbamine,tetrahy-dropalmatine,berberine hydrochloride,palmatine hydrochloride and dehydrocorydaline) in corydalis yanhusuofrom the products and processed products.[Methods] The separationwas performed on an ACQUITY UPLCBEH C18 (100 mm×2.1 mm,1.7 μm) columnin the UPLC method. The mobile phases consisted of acetonitrile (B) and 0.2% formic acid aqueous solutionwith gradient elution. The flow rate was 0.3 mL/min,the column temperature was 55℃,and the detection wavelength was 280 nm.[Results] Good linearities oftetrahydrocolumbamine,protopine,tetrahydrocoptisine,columbamine,tetrahydropalmatine,berberine hydrochloride,palmatine hydrochloride and dehydrocorydaline were achievedwithin the range of 0.395~98.8、0.4~100.2、0.99~247.5、0.197~49.4、1.004~251、0.198~49.5、0.398~99.6、1~250 μg/mL,respectively. The average recoveries were in the range of 98.10%-100.87%,RSD ≤ 1.94 (n=6).[Conclusion] The method established by UPLC is simple,rapid and accurate,and it can be applied to the determination of various components in Corydalis yanhusuo,and provide a more scientific and convenient method for the quality control of Corydalis yanhusuo. |
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