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| Study on the pharmacokinetics of Shengma Gegen decoction granule in beagle dogs by LC-MS/MS |
| Hits 1497 Download times 1224 Received:October 25, 2018 |
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| DOI
10.11656/j.issn.1673-9043.2019.01.18 |
| Key Words
Shengma Gegen decoction granule;LC-MS/MS;beagle dogs;pharmacokinetics |
| Author Name | Affiliation | E-mail | | CHANG Mengchun | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | LIU Jingjing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | LIU Boying | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | REN jing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | ZHANG Lina | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | LI Jiawei | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | lijiawei1981@163.com | | LIU Zhidong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] LC-MS/MS was developed for the determination of the pharmacokinetics of puerarin,paeoniflorin and isoferulic acid in dogs after oral Shengma Gegen decoction granule.[Methods] The column of Waters ACQUITY UPLC® BEH C18(1.7 μm,2.1 mm×50 mm)and a mixture of 0.05% formic acid water solution (A)-acetonitrile(B) as the mobile phase at the flow-rate of 0.2 mL/min,the column temperature was 25℃ and the injection volume was 5 μL.Tolbutamide was used as the internal standard,detection was by negative ion electrospray ionization (ESI) mass spectrometry with multiple-reaction monitoring (MRM). Detection of ion pairs respectively are m/z 415.2→294.9 for puerarin,m/z 525.0→449.0 for paeoniflorin,m/z 193.05→133.9 for isoferulic acid,m/z 269.09→169.9 for tolbutamide.[Results] For the puerarin,paeoniflorin and isoferulic acid,the linear calibration curves were obtained over the concentration range 10~4 000 ng/mL.Endogenous substances in the plasma do not interfere with the determination,the recovery rate,matrix effect,precision,accuracy and stability of the method are in accordance with the requirements of biological samples.[Conclusion] The method has the advantages of strong accuracy,good specificity and high sensitivity,and can be used to detect the pharmacokinetics of puerarin,paeoniflorin and isoferulic acid. |
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