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| Mechanism of Bushen Huoxue decoction in inhibiting podocyte injury and EMT induced by diabetic nephropathy |
| Hits 1425 Download times 1353 Received:January 30, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.03.15 |
| Key Words
Bushen Huoxue decoction;diabetic nephropathy;podocyte injury;Rac1 signaling pathway;EMT |
| Author Name | Affiliation | E-mail | | HU Wei | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | | | XIONG Dan | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | Xiongdan818@163.com | | HUANG Juan | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | |
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| Abstract
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| [Objective] To observe the effect of Bushen Huoxue Decoction on high glucose-induced podocyte injury and epithelial mesenchymal transition(EMT) in mice, and to explore its mechanism of podocyte injury and EMT. [Methods] The immortalized mouse podocyte line MPC5 was cultured in vitro. The cells were randomly divided into control group, high glucose group and Bushen Huoxue Decoction+high glucose group. After corresponding treatment for 48 h, the groups were detected by MTT assay. The proliferation of podocytes was detected by flow cytometry. The migration ability of podocytes in each group was detected by Transwell and scratch test. Reverse transcription polymerase chain reaction(RT-PCR) was used to detect each. The relative expression levels of des-min and FSP-1 in the process of P-cadherin, ZO-1 and EMT were detected. Rac1 inhibitor NSC23766 or Rac1 activator PMA was added to high glucose-induced podocytes, and GTP-Rac1, p-PAK1, p-p38, p-β-catenin and p65 proteins were detected by Western blot in podocytes of different treatment groups. The expression level. [Results] Compared with the control group, high glucose treatment inhibited podocyte proliferation, induced podocyte apoptosis, and promoted cell migration and EMT. The difference is statistically significant (P<0.05). Bushen Huoxue Decoction can alleviate the proliferation inhibition of podocytes induced by high glucose, reduce the damage, apoptosis and migration induced by high glucose, and reverse the occurrence of EMT. The difference is statistically significant(P<0.05). Bushen Huoxue Decoction can affect the activation of Rac1 and its downstream signaling pathway by regulating the expression levels of GTP-Rac1, p-PAK1, p-p38, p-β-catenin and p65. [Conclusion] High glucose can cause podocyte injury and EMT in mice. Bushen Huoxue Decoction can inhibit podocyte injury and EMT induced by high glucose. The mechanism of action is that Bushen Huoxue Decoction protects Rac1 and downstream signaling pathways. The role of podocyte injury. |
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