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| Study on the chemical constituents and effect on platelet aggregation of Chlorophytum tuberosum |
| Hits 1768 Download times 1494 Received:February 02, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.03.19 |
| Key Words
Chlorophytum;chemical constituents;structure identification;platelet aggregation |
| Author Name | Affiliation | E-mail | | XIE Yan | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FU Aizhen | Zaozhuang Mining Group Central Hospital, Zaozhuang 277000, China | | | LI Nan | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Xiaoming | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Peng | Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | zhp8270@sina.com |
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| Abstract
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| [Objective] To investigate the chemical constituents from 50%(v/v) ethanol extract of C. tuberosum and their effect of platelet aggregation activity. [Methods] The chemical constituents were isolated and purified through various columns chromatography and PHPLC. Their structures were identified by NMR and LC-MS spectra. The different polar parts and compounds isolated from C. tuberosum were tested by in vitro anti-platelets aggregation assay. [Results] Eleven compounds were isolated and identified as neogitogenin(1), (25S)-manogenin(2), neohecogenin (3), neohecogenin-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-βD-galactopyranoside (4), neogitogenin-3-O-β-D-glucopy ranosyl-(1 →2)-[β-D-glucopyranosyl-(1 →3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(5), neogitogenin-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl (1→4)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(6), neogitogenin-3-O-β-D-xylopyranosyl-(1→2)-[βD-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (7), neogitogenin-3-O-β-D-xylopyranosyl (1 →3) -[α-L-arabinopyranosyl (1 →2)]-β-D-glucopyranosyl (1 →4)-[α-L-rhamnopyranosyl (1 →2)]-β-Dgalactopyranoside (8), 26-O-β-D-glucopyranosyl-22-hydroxy-25 (S) -5α-furostan-12-oxo-3β, 26-diol-3-O-β-D-xylopyranosyl(1→3)[α-L-arabinopyranosyl(1→2)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-Dgalactopyranoside(9), β-sitosterol(10), daucosterol(11). [Conclusion] Compound 2 was isolated from the Liliaceae for the first time, Compound 1 was isolated from the genus Chlorophytum for the first time and the other compounds were isolated from this species for the first time. The platelet aggregation activity of the different polar parts and compounds were tested by in vitro anti-platelets aggregation, The result indicated that the 95% part had the potent bioactivity against ADP-induced platelet aggregation, however the 70% part had the bioactivity of inducing platelet aggregation. |
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