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| Characterization and in-vitro cellular uptake evaluation of solid lipid nanoparticles loaded with echinacoside |
| Hits 2230 Download times 1695 Received:December 20, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.03.20 |
| Key Words
Echinacoside;solid lipid nanoparticles;HCEpiC;SRA01/04;cellular uptake |
| Author Name | Affiliation | E-mail | | XUE Zhifeng | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | ZHANG bing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | CHEN Jing | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | WU Yumei | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | LU Peng | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | ZHANG Yongmiao | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | QI Dongli | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | LIU Zhidong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 301617, China | lonerliuzd@163.com |
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| Abstract
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| [Objective] The physical characteristics and the cellular uptake in vitro of echinacoside solid lipid nanoparticles (ECH-SLN) optimized by our laboratory were evaluated, which laid a foundation for the improvement of eye preparations in the future. [Methods] ECH-SLN was prepared by emulsion-solidification according to the preoptimized prescription of the laboratory. The physical properties were characterized by differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). Human corneal epithelial cells (HCEpiC) and human lens epithelial cells (SRA 01/04) were selected to analyze the cytotoxicity and cellular uptake of Rhodamine 123 solid lipid nanoparticles(Rh 123-SLN) in vitro. [Results] The DSC results showed that a melting endothermic characteristic peak of ECH at 150℃ was exhibited in the ECH and the physical mixture(blank SLN:ECH=5:1). And the two melting ECH characteristic peaks of SLN appeared at 110℃ and 230℃ in blank SLN, ECH-SLN and physical mixture;The XRD results indicated that a significant ECH peak at 20° was showed in the ECH and the physical mixture. And the characteristic peaks of SLN in the range of 20°~25° were emerged in the blank SLN, ECH-SLN and physical mixture;The results both indicated that ECH was encapsulated in solid lipid nanoparticles (SLN) as a drug in a molecularly dispersed state. The results of cell viability effect showed there was no significant toxicity to both cells for the Rh 123-SLN concentrations of 5.13 μg/mL and 10.25 μg/mL, which was compared with the control group. The cellular uptake experiments showed that the Rh 123 solution could not be taken up by cells, and the cell uptake of Rh 123-SLN was positively correlated with drug concentration and incubation time. [Conclusion] ECHSLN can be delivered the ECH to ocular cells, which may provide an effective drug delivery system for the treatment of oxidative cataract. |
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