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| Experimental research on growth and metastasis of transplanted tumor in mice with breast cancer treated with Yi'ai Fuzheng No. Ⅱ Recipe |
| Hits 1462 Download times 1075 Received:February 18, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.04.16 |
| Key Words
breast cancer;Yi'ai Fuzheng No.Ⅱ recipe;heparan sulfate proteoglycan |
| Author Name | Affiliation | E-mail | | AI Wang | Zhongnan Hospital of Wuhan University, Wuhan 430071 | | | ZHOU Zhen | Zhongnan Hospital of Wuhan University, Wuhan 430071 | | | WANG Xiuping | Zhongnan Hospital of Wuhan University, Wuhan 430071 | | | ZHANG Manling | Hubei University of Traditional Chinese Medicine, Wuhan 430065, China | | | YANG Haitao | Hubei University of Traditional Chinese Medicine, Wuhan 430065, China | | | ZHANG Yingwen | Zhongnan Hospital of Wuhan University, Wuhan 430071 | wangshen@163.com |
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| Abstract
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| [Objective] To observe the inhibitory effect of Yi'ai Fuzheng No.Ⅱ recipe on tumor growth and lung metastasis in breast cancer xenografts in mice, and to explore the relationship between its anti-tumor effect and heparan sulfate proteoglycan.[Methods] The mice model of 4T1 breast cancer was established and randomly divided into blank group, model group, low molecular weight heparin group, high-, medium-, and low-dose groups of Chinese medicine. After 14 days of continuous administration, the samples were sacrificed. The tumor mass and the number of lung metastatic nodules were measured, and the tumor mass inhibition rate and lung metastasis inhibition rate were calculated. The expression of HSPG in transplanted tumor, metastatic tumor and serum of mice in each group was detected by Elisa method.[Results] The tumor quality and the number of lung metastatic nodules of the Chinese medicine group decreased with the increase of dose (P<0.05). The tumor mass inhibition rate and lung metastasis inhibition rate of the high dose Chinese medicine group were better than those of the low molecular weight heparin group (P<0.05). The expression of HSPG in transplanted tumor, metastatic tumor and serum of the Chinese medicine group increased with the increase of dose (P<0.05). The expression of HSPG in transplanted tumor, metastatic tumor and serum of high dose Chinese medicine group was higher than that of low molecular weight heparin group (P<0.05).[Conclusion] The Yi'ai Fuzheng No.Ⅱ recipe can significantly inhibit the proliferation and lung metastasis of transplanted tumor in mice with breast cancer, and its mechanism may be related to the increased expression of heparin sulfate proteoglycan. |
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