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| Protective effect of safflower yellow pigment on periventricular leukomalacia of ischemic rats and its mechanism |
| Hits 1427 Download times 981 Received:March 05, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.04.17 |
| Key Words
safflower yellow pigment;periventricular leukomalacia;newborn rats;brain protection;mechanism |
| Author Name | Affiliation | | LI Peipei | Pharmacy department of puyang hospital of traditional Chinese medicine, Puyang 457000, China | | PENG Jinxia | Department of Burn and plastic surgery, people's Hospital affiliated to Hubei Medical College, Shiyan 442000, China |
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| Abstract
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| [Objective] To investigate the cerebral protective effect and mechanism of safflower yellow pigment on neonatal mice with periventricular leukomalacia (PVL).[Methods] Sixty healthy and clean newborn 7-day old rats were randomly divided into sham operation group, model group, positive control group and safflower groupeach (each 15). In the sham operation group, the common carotid arteries on both sides were dissociated and sutured without ligation, no medication was given. PVL model of newborn mice were built in other groups. The positive control group was given recombinant erythropoietin (rEPO) 5 000 IU/kg intraperitoneal injection immediately after successful modeling. 3 mg/kg safflower yellow pigment was immediately given intraperitoneal injection in the safflower group after successful modeling.The model group was not given medication. After 48 h, rats were sacrificed and the pathological changes of brain tissues were analyzed by HE staining. The contents of SOD, CAT, GPx, MDA and GSH were determined by spectrophotometry and immunohistochemical method was used to detect the expressions of O4, MBP, CX47 and nestin, markers of white matter injury around the ventricle of each group. The expression of Bax, bcl-2, cleaved caspase-9 and cleaved caspase-3 were detected by western blot.[Results] Compared with the model group, the level of the whole brain white matter damage in the positive control group and the safflower group was significantly lower than that in the model group (P<0.05). The number of O4, MBP and CX47 positive cells in the model group was significantly lower than that in the sham group, and the number of Nestin positive cells was significantly higher than that in the sham group. (P<0.05). Compared with the model group, O4, MBP, CX47 and Nestin positive cells were significantly increased in the positive control group and the safflower group (P<0.05). GPx, SOD, CAT and GSH levels in the model group were significantly lower than those in the sham group, and MDA levels were significantly higher than those in the sham group (P<0.05). Compared with the model group, GPx, SOD, CAT and GSH levels in the positive control group and safflower group were significantly increased, while MDA levels were significantly decreased (P<0.05). Compared with the sham group, the expression of Bax was significantly increased, the expression of bcl-2 was significantly decreased, and the activation of Cleaved caspase-9 and Cleaved caspase-3 were significantly increased in the model group (P<0.05). Compared with the model group, the expression of Bax was significantly decreased and Bcl2 expression was significantly increased in the positive control group and safflower group, the activation of Cleaved caspase-9 and Cleaved caspase-3 was significantly reduced (P<0.05).[Conclusion] Safflower yellow pigment can protect the white matter around the ischemic ventricle in newborn rats. The mechanism is not only related to the regulation of antioxidant system, but also may be related to the regulation of apoptosis related factors. |
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