|
| Effect of Naoxintong capsule on wound healing in typeⅡ diabetic mice |
| Hits 1368 Download times 1226 Received:February 19, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2019.04.21 |
| Key Words
NXT;angiogenesis;wound healing;DFU;PI3K/AKT/eNOS signaling pathway |
| Author Name | Affiliation | E-mail | | SONG Min | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Lu | Tianjin University of Traditional Chinese Medicine, Institute of Chinese Medicine, Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin 301617, China | | | LI Chunxiao | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FANG Zhirui | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Lusha | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | Joel Wake Coffie | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Liyuan | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MA Lulu | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Hong | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wanghongsys@tjutcm.edu.cn |
|
| Abstract
|
| [Objective] To study the effect and mechanism of Naoxintong capsule (NXT) on wound healing in type 2 diabetic mice.[Methods] 8~12 weeks db/+ mice and db/db mice were randomly divided into db/+ Saline group, db/db Saline group and db/db NXT (0.7 g/kg). The splint model of excision wound was established. Oral administration and body weight was recorded every day. The fasting blood glucose was monitored and the wound area was measured by photography every two days. At days 7, 16 post-injury, the skin tissues were obtained and the skin tissues at the wounds of mice were sliced or cryopreserved immediately. HE staining and Lectin immunofluorescence was employed to evaluate granulation tissue formation, re-epithelialization and capillaries density around the wound, Western Blotting was used to measure the expression of VEGF, PI3K/P-PI3K, AKT/P-AKT and eNOS/P-eNOS at the edge of the wound.[Results] Compared with the db/db Saline group, the wound healing rate, granulation tissue formation and re-epithelialization, the expression of vascular density and vascular endothelial growth factor increased notably, and the phosphorylation of PI3K/AKT/eNOS increased in db/db NXT group, body weight and fasting blood glucose hadn't change in process of wound closure.[Conclusion] Naoxintong capsule can significantly accelerate wound healing and increase the granulation tissue formation, re-epithelialization and angiogenesis in type 2 diabetic mice by activating PI3K/AK/eNOS signaling pathway. |
|
|
|
|
|
