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| Protective effect of sweroside on heart failure induced by excessive isoproterenol in mice |
| Hits 2033 Download times 1408 Received:May 25, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.05.14 |
| Key Words
sweroside;heart failure;ATPase Na+/K+ Transporting Subunit Alpha 1;energy metabolism |
| Author Name | Affiliation | E-mail | | WANG Qing | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | SU Congping | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | ZHANG Huimin | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | LUO Hui | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | JIAO Wenchao | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | LI Lin | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | | | ZHANG Jingxuan | Beijing University of Traditional Chinese Medicine, Beijing Institute of Traditional Chinese Medicine, Beijing 100029, China | | | GUO Shuzhen | Beijing University of Traditional Chinese MedicineCollege of Traditional Chinese Medicine, Beijing 100029, China | guoshz@bucm.edu.cn |
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| Abstract
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| Heart failure is the serious end-stage of many cardiovascular diseases. Qishen granule has a definite therapeutic effect on heart failure,and the sweroside may be one of the active ingredients to exert its pharmacodynamic effect. The aim of this study was to evaluate the protective effect of sweroside on heart failure induced by excessive isoproterenol in mice,and to explore its potential target and mechanism. Heart failure mice model was established by subcutaneous injection of excessive isoprenaline. Mice were randomly divided into four groups,including control group,model group,sweroside group and captopril group. Cardiac function was evaluated by echocardiography,myocardial pathological changes were detected by HE staining,drug targets were predicted by BATMAN-TCM database,and protein expression of the potential target was detected by Western blot. Compared with the control group,EF and FS of mice in the model group were significantly decreased (P<0.01),while LVIDs was significantly increased (P<0.01). EF and FS were increased in mice treated by sweroside,and LVIDs were decreased (P<0.01). In Histopathological examination,thinned left ventricular wall,thinned myocardial fiber, scattered necrosis of myocardial cells,as well as aggregation of inflammatory cells were found in model mice. The morphology of myocardial tissue were obtained approximate normal by sweroside. BATMAN-TCM analysis showed that ATP1A1 is the candidate target of sweroside in heart failure. Western blot results showed that the expression of ATP1A1 was rescued by sweroside. In this study,by combining of basic pharmacology and network pharmacology, we reported that sweroside significantly improved ISO-induced heart failure in mice through increasing ATP1A1 and then improving myocardial energy metabolism. |
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