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| UPLC fingerprint of Epimedium herbs |
| Hits 1572 Download times 855 Received:March 05, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2019.05.17 |
| Key Words
epimedium;UPLC;fingerprint |
| Author Name | Affiliation | E-mail | | YIN Yanwei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Qi | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Wenjuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | OUYANG Huizi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yanxu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hejun673@163.com |
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| Abstract
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| [Objective] Establishing UPLC fingerprint analysis method for Epimedium medicinal materials.[Methods] Gradient elution with a CORTECS® C18 column (2.1 mm×100 mm,1.6 μm) in acetonitrile-0.1% formic acid water using an Agilent 1 290 series ultra performance liquid chromatography with a flow rate of 0.3 mL/min and a column temperature of 40℃,the injection volume is 1 μL,and the detection wavelength is 270 nm.[Results] There are 21 common peaks in 12 batches of different kinds of epimedium medicinal herbs,including 6 known peaks,namely magnoflorine,epimedin A,epimedin B,epimedin C,icariin and baohuoside I,the similarity between different batch samples and standard fingerprints is above 0.911.[Conclusion] The UPLC fingerprint analysis method established in this experiment is stable and has high precision,and can be applied to the quality control and evaluation of epimedium. |
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