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| Preliminary study on the method of preparing cardiorenal syndrome rat model by 5/6 nephrectomy combined with ISO |
| Hits 1369 Download times 905 Received:November 20, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2020.02.18 |
| Key Words
5/6 nephrectomy;isoproterenol;rat;cardiorenal syndrome;model |
| Author Name | Affiliation | E-mail | | HU Wenzhong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Yun | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Guangya | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Tongyan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | JIANG Nan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | GAO Changbai | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | 18302291547@163.com |
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| Abstract
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| [Objective] Referring to the existing literature on rat models of cardiorenal syndrome,we preparing rat model of cardiorenal syndrome by 5/6 nephrectomy. [Methods] Twenty male SD rats were randomly divided into two groups,the blank group and the operation group. The rats in the blank group were not treated. All rats in the operation group underwent 5/6 nephrectomy according to "two operations". After one week of operation,isoprenaline (2.5 mg/kg,1.5 mg/kg) was injected subcutaneously for two consecutive days (24 h interval) to establish the model of cardiorenal syndrome. The levels of serum creatinine (Cr),serum urea nitrogen (BUN) and brain natriuretic peptide (BNP) were measured in the two groups. The body mass of rats before,after and 6 weeks after model establishment was compared. Six weeks after the establishment of the model,the rats of the two groups were killed,and the heart mass was weighed,and the heart mass index (heart mass/rat body mass) was calculated. The pathological changes of heart and kidney were observed. [Results] After 5/6 nephrectomy combined with subcutaneous injection of isoproterenol in rats,there were significant differences in BNP and Cr levels between the blank group and the operation group (P<0.05). The rats in the operation group had obvious asthma after exercise. For example,the auricle is pale,the coat is sparse and dry and grows slowly. Compared with the blank group,the body weight of the two groups was significantly lower (P<0.05). The pathological changes of heart and kidney were significant differences. [Conclusion] 5/6 nephrectomy combined with ISO subcutaneous injection is a simple and efficient method for the establishment of rat models of cardiorenal syndrome. It can provide a new way for us to further explore the mechanism of the occurrence and drug treatment. However,this method does not fully simulate the natural course of heart failure caused by chronic renal failure. It may not be suitable for studying the early development of the disease,but it is more suitable for evaluating the drug treatment and disease mechanism of cardiorenal syndrome. |
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