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| Experimental study on reversal of early liver cirrhosis by promoting blood circulation and removing blood stasis by multimodal ultrasound |
| Hits 1329 Download times 794 Received:December 23, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2020.02.19 |
| Key Words
ultrasound;early liver cirrhosis;reversal;Fuzheng Huayu Capsule;Xuefu Zhuyu Capsule |
| Author Name | Affiliation | E-mail | | ZHAO Shaoli | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | | | WANG Wenhong | Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | wangwenhong2014@126.com | | FENG Lingling | Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | | | ZHAO Yumeng | Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | | | SUN Jiwei | Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | | | WANG Huan | Department of Radiology, Tianjin Union Medical Center, Tianjin 300121, China | |
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| Abstract
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| [Objective] To explore the application value of multimodal ultrasound technology in evaluating the effect of promoting blood circulation and removing blood stasis on the reversal of early liver cirrhosis in rats. [Methods] Thirty nine Wistar male rats were randomly divided into normal group and model group. The latter was intraperitoneally injected with 0.5% dimethylnitrosamine at 2 μL/g body weight for 4 weeks to replicate the rat early liver cirrhosis model. After successful modeling,they were randomly divided into:model group,Fuzheng Huayu group (FZ group),Xuefu Zhuyu group (XF group) and Fuzheng + Xuefu group (FZ+XF group),the latter three groups were respectively treated by gavage for 4 weeks. After 8 weeks,ultrasound examination was performed to observe relevant parameters such as hepatic parenchymal perfusion and portal vein blood flow,and the correlation between the ultrasound parameters of each group and the treatment effect was analyzed. After the rats were sacrificed,blood was collected through the abdominal aorta and tested for serological indicators;liver tissues were fixed and stained with HE and Masson. [Results] 1) In the model group,the diameter of portal vein increased,the velocity of blood flow slowed down,the intensity of peak parenchyma decreased,the peak time prolonged,the area under the curve decreased,the content of serum hyaluronic acid (HA),laminin (LN),procollagen Ⅲ (PC Ⅲ) and collagen Ⅳ (C Ⅳ) increased,there was a significant difference between the model group and the control group (P<0.05). 2) In the FZ group,XF group and FZ + XF group,the internal diameter of the portal vein decreased,the flow velocity increased,the peak parenchymal intensity of the liver increased,the peak time decreased,and the area under the curve increased. Compared with the model group,there were significant differences (P<0.05). 3) The portal vein diameter of rats in the FZ + XF group decreased,and the peak time of liver parenchyma in the FZ + XF group and the XF group slowed down,which was significantly different from that in the FZ group (P<0.05). 4) The serum LN,type III procollagen,type IV collagen levels of three groups and the serum HA levels of the FZ and FZ + XF groups in the three treatment groups were significantly lower than those in the model group (P<0.05). 5) HE and Masson staining of liver parenchyma showed that the treatment of promoting blood circulation and removing blood stasis can improve the degeneration and necrosis of liver cells,inhibit the proliferation of collagen fibers,and reduce the degree of liver fibrosis. [Conclusion] After treatment with three groups of drugs,portal hypertension in rats improved and liver blood perfusion increased,and the FZ+XF and XF groups were significantly better than the FZ group. Compared with pathology,the treatment of promoting blood circulation and removing blood stasis was helpful to the early liver cirrhosis in rats. reverse. The use of multi-modal ultrasound technology to evaluate the effect of activating blood circulation and removing blood stasis on reversing early liver cirrhosis in rats has certain application value. |
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