|
| Content determination of saikosaponins in Bupleuri Radix from different habitats |
| Hits 1289 Download times 848 Received:December 02, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2020.02.22 |
| Key Words
Bupleurum chinense DC;Bupleurum scorzonerifolium Willd;saikosaponin a;saikosaponin c;saikosaponin d;saikosaponin f;saikosaponins;content determination |
| Author Name | Affiliation | E-mail | | GUO Tailin | College of pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116001, China | | | KANG Tingguo | College of pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116001, China | | | ZHANG hui | College of pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116001, China | syyycs@163.com |
|
| Abstract
|
| [Objective] To determine total saponins and four monomers saponins (saikosaponin a,d,c and f) in Bupleuri Radix from different habitats. [Methods] Using saikosaponin d as the standard,the total saponins in 24 batches of B. chinense DC. and 5 batches of B. scorzonerifolium Willd. were determined at 536 nm by UV-Vis spectrophotometry. The content of saikosaponin a,d,c and f were determined by HPLC-ELSD. [Results] The content of saikosaponin d in the same batch of samples was generally higher than that of saikosaponin a. There were significant differences in the contents of saikosaponin a,c,d and f from different habitats. There was a good linear relationship in range of 0.08~0.4 mg (r=0.999). The recovery of sample addition ranged from 99.2% to 103%,RSD=1.42%. The content of total saponins ranged from 5.32% to 13.9%,and the content of total saponins in B. scorzonerifolium Willd. was higher than that in B. chinense DC. The content of total saponins in B. scorzonerifolium Willd. from Linyi (Shandong Province) was the highest,reaching 13.9%. [Conclusion] The quality evaluation of Bupleuri Radix can not only determine the content of saikosaponin a,it should determine saikosaponins and saikosaponin a,c,d and f at the same time for more accurately. |
|
|
|
|
|
