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| Determination and release in vitro of polyphyllin VI in ChongHuang thermosensitive in situ gel |
| Hits 1215 Download times 767 Received:December 20, 2019 |
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| DOI
10.11656/j.issn.1673-9043.2020.02.23 |
| Key Words
Chonghuang thermosensitive in situ gel;polyphyllin VI;in vitro release;HPLC-ELSD |
| Author Name | Affiliation | | SUN Yuan | Yunnan Institute of Materia Medica, Kunming 650111, China
Yunnan Baiyao Group Innovation and R&D Center, Kunming 650111, China
Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China | | DING Jiangsheng | Yunnan Institute of Materia Medica, Kunming 650111, China
Yunnan Baiyao Group Innovation and R&D Center, Kunming 650111, China
Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China | | WAN Jinfu | Yunnan Institute of Materia Medica, Kunming 650111, China
Yunnan Baiyao Group Innovation and R&D Center, Kunming 650111, China
Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China |
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| Abstract
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| [Objective] To establish a determination method for the content of polyphyllin VI in ChongHuang thermosensitive in situ gel and to study the release in vitro. [Methods] A HPLC-ELSD method was adopeted with the CAPCELL PAK C8 column (4.6 mm×250 mm,5 μm),with the mobile phaseconsisting of acetonitrile(A)-water(B)at the flow rate of 1.0 mL/min (0~15 min,25%~35% A;15~35 min,35%~40%A;35~40 min,40%~60%A),with the column temperature 25℃,with the injection volume 10 μL,with the drift tube temperature 92℃ and the carrier gas pressure was 2.9 L/min. [Results] The polyphyllin VI has good linearity in the range of 0.430 0~4.300 0 mg,lgA=1.796 9lgC+4.398 9(R2=0.999 9),The average recovery rate was 101.70% (RSD=1.17%,n=9). The in vitro release study indicated that drug release of polyphyllin VI was best fitted to Higuchi equation. [Conclusion] The established method is proved to be convenient,stable and efficient. |
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