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| Mechanism of Zhenwu Decoction and Linggui Zhugan Decoction in improving water-sodium retention in rat model of cardiorenal syndrome |
| Hits 1374 Download times 884 Received:January 25, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.03.15 |
| Key Words
Zhenwu Decoction and Linggui Zhugan Decoction;cardiorenal syndrome;rats;aquaporin 2;isoproterenol;5/6 renectomy |
| Author Name | Affiliation | | GAO Changbai | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | HU Wenzhong | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | JIANG Nan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | WANG Baojuan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | YANG Meijuan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | XU Yun | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China |
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| Abstract
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| [Objective] To study the mechanism of Zhenwu Decoction and Linggui Zhugan Decoction on water and sodium retention in rats with cardiorenal syndrome.[Methods] The 60 SD rats were randomly divided into two groups,the blank group and the operation group. Cardiorenal syndrome model was established by 5/6 nephrectomy combined with subcutaneous injection of isoproterenol in rats of operation group. After eliminating the dead rats,the remaining rats in the operation group were randomly divided into model group,traditional Chinese medicine group (TCM group) and conventional treatment group according to body weight. Rats in the TCM group were received 7.29 g crude drug/(kg·d)for intragastric administration. The routine treatment group was given benazepril[0.45 mg/(kg·d)] and furosemide[1.8 mg/(kg·d)]. The blank group and model group were intragastrically administered with normal saline of equal volume. They were given once a day for 6 consecutive weeks. The mental state,activity,sensitivity,hair condition,appetite,urine and feces and mortality of rats in each group were observed during the experiment. The difference of serum brain natriuretic peptide (BNP),serum creatinine (Scr),serum urea nitrogen (BUN) and urinary aquaporin 2 (AQP2) before and 6 weeks after gastric administration were compared.[Results] After 6 weeks of intragastric administration,the general situation of rats in the TCM group was better than that before gastric administration,and the wheezing was not obvious. The general situation of rats in the conventional treatment group was also better than that before gastric administration. By comparing the difference of laboratory indexes of rats before and after treatment,it was found that BNP in the TCM group and the conventional treatment group decreased,and the improvement of heart failure in the TCM group was close to the level of the conventional treatment group. There was no significant difference between the two groups (P>0.05). The level of Scr in TCM group was lower than before,while that in the conventional treatment group was higher. The level of AQP2 in urine of TCM group was slightly higher than before. There was no significant difference in urinary AQP2 level between TCM group and blank group (P>0.05). The level of urinary AQP2 in routine treatment group was significantly higher than that before.[Conclusion] The expression of urinary AQP2 in rats with Cardiorenal Syndrome is mainly regulated by the kidney,AQP2 can be used as a reference index for water and sodium retention in patients with cardiorenal syndrome. Zhenwu Decoction and Linggui Zhugan Decoction can improve the state of water and sodium retention in rats with cardiorenal syndrome. It may be through inhibiting the excessive expression of AQP2 in the kidney of rats, reducing the reabsorption of water and improving the state of water and sodium retention. |
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