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| Study on the mechanism of Huanglong Zhike Oral Liquid on mouse with cough variant asthma model |
| Hits 1390 Download times 802 Received:February 02, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.03.18 |
| Key Words
Huanglong Zhike Oral Liquid;cough variant asthma;airway inflammation;Toll-like receptors;NF-κB |
| Author Name | Affiliation | E-mail | | WANG Chunyan | Graduate School of Nanjing University of Chinese Medicine, Nanjing 210029, China | | | LONG Hongyan | Department of Pediatrics, Nanjing Hospital of Chinese Medicine, Nanjing 210023, China | hongyan3128@163.com |
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| Abstract
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| [Objective] To investigate the possible mechanism of Huanglong Zhike Oral Liquid in the treatment of cough variant asthma.[Methods] Fifty BALB/c mice were randomly divided into control group,the model group,dexamethasone (Dex) group and Huanglong Zhike Oral Liquid low and high dose groups,with 10 mice in each group. The mice model of cough variant asthma was established by intraperitoneal injection of ovalbumin and aluminum hydroxide,sensitization and inhalation of ovalbumin. The mice in Huanglong Zhike Oral Liquid group were given 0.75g/kg and 1.5g/kg by gavage respectively once a day. The mice were killed after 14 days.Serum was collected and the levels of total IgE and interleukin-4 (IL-4),IL-5 and IL-13 were detected by ELISA. Lung tissues of mice were extracted and observed by HE staining. Toll-like receptors 4 (TLR4),myeloid differentiation factor 88 (MyD88)and nuclear factor-κB (NF-κB) were detected by Western blot.[Results] Compared with the control group,the levels of inflammatory cells,IgE,IL-4,IL-5 and IL-13 in the serum of the model group were significantly increased,and the expressions of TLR4,MyD88 and NF-κB in the lung tissue were significantly increased (P<0.01). Compared with the model group,the pathological sections of the Dex group,Huanglong Zhike Oral Liquid low-dose group and high-dose group were improved,the content of inflammatory cells and inflammatory factors in serum,the expression of TLR4,MyD88 and NF-κ B protein in lung tissue were decreased (P<0.01).[Conclusion] Huanglong Zhike Oral Liquid can improve airway inflammation and airway hyperresponsiveness in cough variant asthma model mice,and its mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signal pathway. |
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