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| The establishment and application of chemical fingerprint and multi-component quantitative analysis for Leonuri Herba |
| Hits 1413 Download times 831 Received:January 20, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.03.19 |
| Key Words
Leonuri Herba;HPLC;fingerprint;quantitative analysis;pattern recognition |
| Author Name | Affiliation | E-mail | | LI Baohui | Deparment of Central Laboratory, 989 th Hospital of the Chinese People's Liberation Army, Luoyang 471031, China | | | LI Donghui | Deparment of Central Laboratory, 989 th Hospital of the Chinese People's Liberation Army, Luoyang 471031, China | | | XUE Dangdang | Deparment of Central Laboratory, 989 th Hospital of the Chinese People's Liberation Army, Luoyang 471031, China | | | ZHAO Guang | Deparment of Central Laboratory, 989 th Hospital of the Chinese People's Liberation Army, Luoyang 471031, China | bjhmli@163.com |
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| Abstract
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| [Objective] To build a novel method for quality control of Leonuri Herba(LH) combining HPLC fingerprint and multi-component quantitative analysis.[Methods] HPLC-DAD-MS was applied to determine 110 LH samples,and HPLC fingerprint was developed based on seven characteristic compounds,where a structure was assigned to five,screened by GA-PLS. Then,quantitative analysis was performed on contents of leonurine hydrochloride,rutin,quercetin,kaempferol,and apigenin,by HPLC external standard methods.[Results] Performance evaluation of HPLC fingerprint:the good identification degree was confirmed by all the four pattern recognition methods,PCA,LDA,LS-SVM,and RBF-NN,while RBF-NN obtained best results with 98.6% recognition rate for calibration set,and 97.3% for prediction set. The quantitative analysis of characteristic components showed LH samples originated from Xinjiang and Henan had high contents for each compound. In additional,similar contents of some compounds were detected in LH samples of different origins.[Conclusion] It is wonderful tools,which combined HPLC fingerprint and quantitative analysis,to discriminate origins and quality evaluate for LH samples. |
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