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| Pathology effect of Polygonum Cuspidatum's effective components on kidneys of mice with diabetic nephropathy |
| Hits 1242 Download times 796 Received:February 04, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.04.17 |
| Key Words
emodin;polydatin;diabetic nephropathy;renal pathology |
| Author Name | Affiliation | E-mail | | WANG Dali | Community Health Service center in Yanshan of Fangshan District, Beijing 102500, China | | | MENG Fengxian | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | SHANG Xuezheng | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | WANG Jie | Shunyi Hospital Affiliated to Beijing Hospital of Traditional Chinese Medicine, Beijing 101300, China | | | ZHANG Chengcheng | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | BAI Hua | Guangwai Hospital of Xicheng District, Beijing 100053, China | | | BU Xiangwei | Traditional Chinese Medicine Department of Xiamen University, Xiamen 361005, China | | | ZHANG Zhengju | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | LIU Yuyue | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | Liuyuyue1985@126.com |
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| Abstract
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| [Objective] To investigated the pathology effect of PolygonumCuspidatum's effective components on kidneys of mice with diabetic nephropathy(DN).[Methods] KK-Ay mice with spontaneous type 2 diabetes were fed with high-fat diets for 3 weeks to establish DN models. The successful models of mice were randomly divided into the model group,the positive drug group,high-dose,medium-dose and low-dose groups of emodin and high-dose,medium-dose and low-dose groups of polydatin. The C57BL/6J mice were recruited as the control group. Each group included 6 mice. Each mice was administered once daily. After twelve weeks of intervention,all mice were sacrificed and the kidneys were harvested for hematoxylin-eosin (HE) and periodic acid-schiff (PAS) stain. The renal lesions were classified and scored under light microscope. The relative area of mesangium extracellular matrix was measured under the PAS stain. Ultrastructural morphological changes of renal tissue were measured under electronmicroscope.[Results] Compared with the model group,the renal tubular lesions in the high dose of polydatin group were significantly reduced (P<0.05);Mesangium extracellular matrix relative area was significantly decreased in polydatin group and the high dose group of emodin (P<0.01,P<0.05). The pathological changes of glomerulus were diminished in emodin group. The structure of podocytefootprocess was normal in the medium dose group of polydatin which can significantly reduce the ultrastructural lesions of renal tissue.[Conclusion] Emodin and polydatin can significantly lighten the renal lesions of mice with DN and have a protective effect on kidney. |
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