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| Effect of Danshen and Ligustrazine Injection on Th17/Treg balance in rats with ischemic stroke |
| Hits 1135 Download times 799 Received:January 10, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.04.18 |
| Key Words
Danshen and Ligustrazine Injection;ischemic stroke;Th17;Treg;nerve injury |
| Author Name | Affiliation | | QUAN Hongjuan | Neurology Department, First People's Hospital of Chenzhou City, Binzhou 423000, China |
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| Abstract
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| [Objective] To investigate the effect of Danshen and Ligustrazine Injection on Th17/Treg balance in rats with ischemic stroke.[Methods] The middle cerebral artery occlusion (MCAO) rat model was made,and the experimental rats were randomly divided into three groups:sham operation group,model group and Shenxiong group. Each group was divided into four phases:24 hours,3 days,7 days and 14 days,10 animals in each phase. The rats in Shenxiong group were injected with 0.9 ml/kg Danshen and Ligustrazine Injection once daily,and the rats in sham operation group and model group were given the same amount of normal saline. At the end of treatment,the neural function of the animals was scored by NSS score,TTC (triphenyltetrazolium chloride) staining method was used to detect the brain infarction volume,and the brain tissue of the blood supply area of the middle cerebral artery was separated. The proportion of Th17 and Treg cells was detected by flow cytometry,and the level of inflammatory cytokines was detected by ELISA.[Results] The NSS scores and the brain infarction volume of model group and Shenxiong group decreased gradually with time,and there were significant differences between them (P<0.01);and from the third day,significant differences were detected in the NSS scores and the brain infarction volume between the model and Shenxiong groups at each time point (P<0.05,P<0.01). The proportion of Th17 cells in the brain of the model group increased gradually after the model was established,and reached the peak on the 7th day,and the proportion on the 3rd,7th and 14th day was significantly higher than that of the sham operation group (P<0.01). The ratio of Treg cells in the model group decreased at 24h and 3D,but increased significantly at 7d (P<0.01). Compared with the model group,the ratio of Th17 cells and Treg cells in Shenxiong group decreased significantly (P<0.05,P<0.01). The ratio of Th17/Treg in the model group was significantly higher than that in the sham operation group (P<0.05,P<0.01),while that in the Shenxiong group was significantly lower than that in the model group (P<0.05,P<0.01). The level of IL-6 and TNF-α in the brain of the model group increased gradually and reached the peak on the 7th day,and the level of each time point was higher than that of the sham operation group,the difference between the groups was significant (P<0.01). The level of IL-4 and IL-10 in model group was lower than that in sham operation group,and the decrease of IL-4 was more obvious (P<0.05). Compared with the model group,Shenxiong group can reduce the level of IL-6 and TNF-α at each time point (P<0.05,P<0.01),and can increase the level of IL-4 and IL-10,especially the level of IL-4 (P<0.05).[Conclusion] Salvia miltiorrhiza and Ligustrazine Injection can regulate the Th17/Treg balance and the level of related inflammatory factors in rats with ischemic stroke,thus improving the microenvironment of neuron survival and alleviating the injury of nerve ischemia. |
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