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| Effect of Bushen Huoxue Decoction on serum cystine C and blood and urine β2 microglobulin in rats with type 2 diabetic nephropathy and its renal protective mechanism |
| Hits 1212 Download times 750 Received:May 21, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2020.05.22 |
| Key Words
type 2 diabetic nephropathy;rat model;Bushen Huoxue Decoction;oxidative stress;serum cystine C;β2-microglobulin |
| Author Name | Affiliation | E-mail | | XIONG Dan | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | | | HU Wei | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | Xiongdan818@163.com | | HUANG Juan | First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | |
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| Abstract
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| [Objective] To investigate the effect of Bushen Huoxue Decoction on serum cystine c (Cys-c) and blood and urine β2 microglobulin (β2-MG) in rats with early type 2 diabetic nephropathy,and to analyze the possible mechanism of renal protection.[Methods] Sixty five rats with type 2 diabetic kidney disease with deficiency of both qi and yin were established by intraabdominal injection of low dose streptozotocin with single kidney resection and high fat diet,combined with behavioral intervention. The patients were randomly divided into three groups:false operation group (n=15),model group (n=15),tonifying kidney and activating blood circulation group (n=20) and benazepril group (n=15). Blood glucose,including fasting blood glucose (FBG) and postmeal 2 h blood glucose (PBG),were measured in each group after intervention. Serum and renal cortex (SOD),malondialdehyde (MDA),blood urine nitrogen (BUN),serum creatinine (Cr),24-hour urinary albumin (24 h-UAlb),Cys-c and blood,urine β2-MG,etc. The pathological characteristics of renal tissue in each group were observed under light microscope.[Results] BUN and Cr increased significantly (P>0.05),FBG,PBG,serum and renal cortex MDA,24 h UAlb,Cysc and blood,urinary β2-MG increased significantly,and the serum and renal cortex SOD decreased significantly (P<0.05 or P<0.01). Compared with the model group,the serum and renal cortex MDA,24 h UAlb,Cysc and blood,urinary β2MG were significantly lower,and the serum and renal cortex SOD were significantly higher (P<0.05). Compared with benazepril group,MDA in serum and renal cortex was lower and SOD was significantly higher in kidney-tonifying and activating blood group (P<0.05),but there was no significant difference in 24-hour UAlb,Cys-c,blood and urine β2-MG(P>0.05),and the degree of renal pathological changes in four groups was false operation group,kidney tonifying and activating blood circulation group,benazepril group and model group.[Conclusion] Bushen Huoxue Decoction can effectively reduce blood glucose,24 h-UAlb,Cys-c,blood and urine β2-MG in rats with type 2 diabetic nephropathy. The mechanism of Bushen Huoxue Decoction may be achieved by improving oxidative stress. |
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